用羧甲基聚合环糊精作手性剂的毛细管电泳法分离3种碱性药物  

The Separation of Three Basic Drugs with Carboxymethyl-Poly- β-Cyclodextrin as Chiral Additive by Capillary Electrophoresis

作　　者：范瑞芳[1] 史雪岩[1] 顾峻岭[1] 邓玉林[1] 傅若农[1] 

机构地区：[1]北京理工大学化工与材料学院,北京100081

出　　处：《色谱》2000年第4期357-360,共4页Chinese Journal of Chromatography

基　　金：国家自然科学基金!资助项目 (No 2 97750 0 6)

摘　　要：采用 5种环糊精衍生物对碱性药物硫喷妥钠、盐酸氟桂利嗪、山梗菜碱进行了毛细管区带电泳的手性拆分。结果表明 ,采用含 2 % (质量分数 ,其余相同 )聚合 β 环糊精 (P β CD)或 0 5%羧甲基聚合 β 环糊精 (CM P β CD) 30mmol/L的Tris H3PO4缓冲液可使这 3种药物达到基线分离 ;使用CM P β CD时 ,分离度高达 4～35。Five cyclodextrin derivatives, namely phosphate ester β CD,carboxymethyl β CD(CM β CD),bis [6 oxygen (β carboxymethyl succinic acid 4 ester)]β CD(F CM β CD),β CD polymer(P β CD),carboxymethyl poly β CD(CD P β CD), were used as chiral selector for separation of three basic drugs, lobeline, thiopendonesodium, flunarizine by capillary zone electrophoresis (CZE) All the five cyclodextrins have chiral separation ability to lobeline P β CD and CM P β CD have chiral recognition to thiopentonesodium and flunarizine The results indicate that via optimizing separation conditions by varying pH and the concentration of chiral selectors the three racemic drugs could be baseline separated with 2% P β CD or 0 5% CM P β CD in the buffer of 30 mmol/L Tris H\-3PO\-4 And the best resolution ranging from 4 to 35 with CM P β CD as chiral additive was obtained within 10 min The results were much better than those reported in other references.

关 键 词：硫喷妥钠 盐酸氟桂利嗪 山梗菜碱 CE 手性拆分 

分 类 号：R914.1[医药卫生—药物化学] O657.8[医药卫生—药学]