云芝菌株胞外多糖的制备及纯化  

Preparation and purification of extracellular polysaccharide from Coriolus versicolor

作　　者：孙静[1] 尤毅[1] 蒋发现[1] 杨锐[1] 蔡俊立[1] 赵林果[1] 

机构地区：[1]南京林业大学化学工程学院,江苏省生物质绿色燃料与化学品重点实验室,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2012年第6期85-89,共5页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：为提高云芝产胞外多糖的产量,在单因素试验的基础上,应用响应面分析法对云芝菌丝体产胞外多糖的培养条件进行了优化。根据回归分析,确定的较佳工艺条件为:培养时间2.4 d,装液量67.6 mL,初始pH=5.1。利用醇沉淀法和DEAE Sepharose CL-6B凝胶柱层析对云芝胞外多糖进行纯化,产品达到电泳纯。紫外扫描和HPLC的分析结果表明,云芝菌株胞外多糖不含多肽,单糖组成至少含有葡萄糖和木糖。To study the optimization of culture conditions on the extracellular polysaccharides Coriolus versicolor,the response surface methodology（RSM） based on the single factor tests were applied.According to regression analysis,the optimal culture conditions were culture time 2.4 days,broth content 67.6 mL,and initial pH 5.1.In order to get the purified polysaccharide,alcohol precipitation and DEAE Sepharose CL-6B purification were used.The polysaccharide was identified the uniform component by PAGE.The HPLC results showed that the components of monosaccharide contain at least glucose and xylose,without peptides.

关 键 词：云芝 胞外多糖 响应面分析法 纯化 

分 类 号：S567[农业科学—中草药栽培] Q943[农业科学—作物学]