电离辐射对MC3T3细胞增殖和Notch1、Jagged1基因表达的影响  

Effects of ionizing radiation on MC3T3 cells' proliferation and expression of Notch1 and Jagged1

作　　者：唐泉[1] 杨冰[1] 仲蕾蕾[1] 杨福军[1] 张晓东[1] 尚杰[2] 王芹[1] 韩英[1] 樊体强[1] 樊飞跃[1] 孙元明[1] 

机构地区：[1]北京协和医学院&中国医学科学院放射医学研究所 天津分子核医学重点实验室,天津300192 [2]天津医科大学总医院药剂科,天津300052

出　　处：《辐射研究与辐射工艺学报》2012年第6期374-377,共4页Journal of Radiation Research and Radiation Processing

基　　金：国家自然基金(30970867);中国医学科学院放射医学研究所所基金(SF1105/ST1223)资助

摘　　要：揭示了电离辐射对成骨细胞系MC3T3细胞的增殖及其对Notch1和Jagged1基因表达的影响。成骨细胞系MC3T3细胞,经过0、2和4Gy ^(137)Csγ射线照射,运用MTT、实时定量PCR技术,检测电离辐射对与MC3T3细胞的增殖和增殖分化相关基因Notch1和Jagged1表达的影响。照射剂量为2和4 Gy时,细胞的增殖下降(p<0.05,p<0.01);照射剂量为2Gy时,Notch1基因表达量降低(p<0.05);照射剂量为2和4Gy时,Jagged1基因的表达量均降低(p<0.01,p<0.05)。电离辐射损伤使成骨细胞系MC3T3的增殖能力下降,同时诱发Notch1基因和Jagged1的低表达。ABSTRACT In order to investigate the effects of ionizing radiation on MC3T3 cells＇ proliferation and expression changes of Notchl and Jaggedl genes, MC3T3 cells were exposed to γ-rays with 0, 2 and 4 Gy, respectively. MTT assay and real-time PCR were adopted to detect the proliferation of MC3T3 cells and the changes of expression of Notchl and Jaggedl genes which could be in association with proliferation and differentiation. When exposed to 2 and 4 Gy γ-rays irradiation, the MC3T3 cells＇ proliferation reduced （p〈0.01, p〈0.01）. The expression of Notchl decreased when treated with 2 Gy irradiation （p〈0.05）. The expression of Jaggedl regulated down when given 2 and 4 Gy irradiation （p〈0.01, p〈0.05）. Irradiated MC3T3 cells＇ proliferation potentialities reduced and Notchl and Jaggedlwere regulated down.

关 键 词：电离辐射 成骨细胞 MC3T3 NOTCH1 JAGGED1 

分 类 号：R336[医药卫生—人体生理学] R811.5[医药卫生—基础医学]