用左旋多巴-示差脉冲伏安法间接测定生物样品中的铝  

Indirect Differential Pulse Voltammetric Determination of Aluminum in Biological Samples with L Dopa

作　　者：章福平[1] 毕树平[1] 刘锋[1] 卞宁生[1] 

机构地区：[1]南京大学化学系,配位化学国家重点实验室,南京210093

出　　处：《高等学校化学学报》2000年第8期1205-1207,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金! (批准号 :4983 10 0 5 ;2 97770 13 );中国科学院长春应用化学研究所电化学开放实验室开放基金资助

摘　　要：Electroanalytic direct determination of aluminum is a difficult subject because of the highly negative reductive potential of Al(Ⅲ) and interference of H +. But developing methods for the determination of trace amount of aluminum in environmental and biological samples is a hot topic in recent decades owning to the recognition of the potential toxic effects of aluminum. In this paper, the indirect differential pulse voltammetric(DPV) determination of aluminum with L dopa(3,4 dihydroxyphenylalanine) on glass carbon working electrode has been reported. This novel method bases on the linear decrease of DPV anodic peak current of L dopa with the concentration of aluminum added. Under the optimum experimental conditions (pH 8 5, 0 08 mol/L buffer solution, 4×10 -4 mol/L L dopa), the linear range and the detection limit are (2—18)×10 -7 and 7 6×10 -8 mol/L Al(Ⅲ) respectively, the relative standard deviation for 2×10 -5 mol/L Al (Ⅲ) is 2 4%(n=10). A number of foreign species, especially those necessary components in human body such as ascorbic acid, citrate, phosphate, Fe(Ⅲ), Fe(Ⅱ), Cu(Ⅱ), Zn(Ⅱ), Ca(Ⅱ) and Mg(Ⅱ) for interference have been studied. Satisfactory recoveries have been obtained by applying this method to determining aluminum in biological samples including mineral water, synthetic renal dialysate, urine and hair. The significance of the method and solutions to possible interference encountered in in vivo detection are discussed. A new direction for further development of the in vivo determination of aluminum is provided in this studies.Electroanalytic direct determination of aluminum is a difficult subject because of the highly negative reductive potential of Al(Ⅲ) and interference of H +. But developing methods for the determination of trace amount of aluminum in environmental and biological samples is a hot topic in recent decades owning to the recognition of the potential toxic effects of aluminum. In this paper, the indirect differential pulse voltammetric(DPV) determination of aluminum with L dopa(3,4 dihydroxyphenylalanine) on glass carbon working electrode has been reported. This novel method bases on the linear decrease of DPV anodic peak current of L dopa with the concentration of aluminum added. Under the optimum experimental conditions (pH 8 5, 0 08 mol/L buffer solution, 4×10 -4 mol/L L dopa), the linear range and the detection limit are (2—18)×10 -7 and 7 6×10 -8 mol/L Al(Ⅲ) respectively, the relative standard deviation for 2×10 -5 mol/L Al (Ⅲ) is 2 4%(n=10). A number of foreign species, especially those necessary components in human body such as ascorbic acid, citrate, phosphate, Fe(Ⅲ), Fe(Ⅱ), Cu(Ⅱ), Zn(Ⅱ), Ca(Ⅱ) and Mg(Ⅱ) for interference have been studied. Satisfactory recoveries have been obtained by applying this method to determining aluminum in biological samples including mineral water, synthetic renal dialysate, urine and hair. The significance of the method and solutions to possible interference encountered in in vivo detection are discussed. A new direction for further development of the in vivo determination of aluminum is provided in this studies.

关 键 词：左旋多巴 铝 示差脉冲伏安法 脑疾病诊断 

分 类 号：R742.04[医药卫生—神经病学与精神病学] O614.31[医药卫生—临床医学]