气相色谱-质谱法同时检测食物中的氟乙酰胺与毒鼠强方法研究  

Simultaneous determination of fluoracetamide and tetramine in food by gas chromatography-mass spectrometry

作　　者：陈蓓[1] 刘华良[1] 荣维广[1] 朱峰[1] 马永建[1] 

机构地区：[1]江苏省疾病预防控制中心,江苏南京210009

出　　处：《中国食品卫生杂志》2012年第6期539-542,共4页Chinese Journal of Food Hygiene

基　　金：卫生部公益性行业科研专项(200902009);"十二五"科教兴卫工程"突发公共卫生事件应急处置创新平台"(ZX201109)

摘　　要：目的建立气相色谱-质谱检测仪同时测定食物中氟乙酰胺与毒鼠强的方法。方法样品用乙腈提取,经DB-WAX弹性毛细管柱(30 m×0.25 mm×0.25μm)色谱柱程序升温分离,采用单四级杆质谱进行测定,以保留时间和特征离子为定性依据,外标法定量。结果氟乙酰胺和毒鼠强的线性范围分别为0.05～10.0 mg/L和0.01～10.0 mg/L,相关系数(r)>0.999;方法的检出限分别为0.04和0.01 mg/kg;氟乙酰胺的加标回收率为60.0%～79.4%,毒鼠强的加标回收率为68.2%～92.5%。结论本法灵敏度高,简便,易行,结果准确可靠,基本满足中毒食物快速检定的要求。Objective To establish a GC-MS method of simultaneous determination of fluoroacetamide and tetramine in food.Methods Target compound was extracted by acetronitrile,then separated by DB-WAX capillary column and detected by single quadrupole mass spectrometry.Retention time and characteristic ions were used as qualitative evidence,and external standard was used for quantification.Results The linear range for fluoroacetamide and tetramine was 0.05-10.0 mg/L and 0.01-10.0 mg/L（r0.999）,the limit of detection was 0.04 mg/kg and 0.01 mg/kg,and the average recoveries was 60.0%-79.4% and 68.2%-92.5%.Conclusion The method is sensitive,convenient,accurate and reliable.It is suitable for rapid determination of fluoroacetamide and tetramine in food.

关 键 词：氟乙酰胺 毒鼠强 鼠药 GC-MS 食品安全 

分 类 号：R155[医药卫生—营养与食品卫生学]