PCR快速诊断急性坏死性胰腺炎继发真菌感染的研究  

Study on rapid diagnosis of fungal infection in the patients with acute necrotizing pancreatitis by polymerase chain reaction

作　　者：张卫中[1] 韩天权[2,3] 汤耀卿 万志明[2,3] 张圣道 

机构地区：[1]浙江省台州市黄岩第一人民医院普外科,318020 [2]上海第二医科大学附属瑞金医院外科 [3]上海消化外科研究所,上海市200025

出　　处：《中国实验诊断学》2000年第3期109-111,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨针对真菌18SrRNA基因的通用引物聚合酶链反应（PCR）技术诊断急性坏死性胰腺炎继发真菌感染的价值。方法建立PCR方法检测常见的临床真菌分离菌株；采用PCR技术和常规培养方法同时检测37份急性坏死性胰腺炎临床标本。结果对于临床真菌分离菌株，该PCR方法扩增出197bp大小的片段，而革兰氏阳性、阴性菌和人血白细胞呈阴性表达。11例急性坏死性胰腺炎患者的37份坏死组织或胰周脓液标本经真菌培养阳性为6份，而经PCR检测阳性为8份，PCR检测时间为7h。结论该PCR方法可以快速、敏感地诊断急性坏死性胰腺炎继发真菌感染。Objective To assess the rapid diagnosis of rungal infection in the patients with acute necrotizing pancreatitisv by Polymerase chain reaction (PCR) using universal primers targeting 18s rRNA gene. Methods PCR assay was developed to identify clinically isolated fungi, and both PCR technique and conventional cultare were used to detect fungi in 37 samples from patients with acute necrotizing pancreatitis. Results By this PCR assay, a 197bp haent was amplified from all the clinically isolated drigal strains, and not from gram-positive, gram-negative bacteria or human leucocytes. When analyaing 37 necrotic tissues or peripancreatic fluids from 11 patients with acute necrotizing pancreatitis, 6 samples were proved positive for fungi by conventional culture and 8 Positive by PCR. Whole PCR procedure was completed within 7 hours. Condndon The PCR techniqure can be used to diagnose the fungal infechon secondaly to actue necrotizing pancreatitis rapidly and sensitively.

关 键 词：急性坏死性胰腺炎 真菌感染 聚合酶链反应 

分 类 号：R576[医药卫生—消化系统]