血性脑脊液对内皮细胞分泌ET和NO的影响  

Effect of BCSF on the Secretion or Endothelin and Nitric Oxide in Endothelial Cells

作　　者：邵国富[1] 张志琳[1] 楚冰[1] 包仕尧[1] 黄强[2] 

机构地区：[1]苏州医学院附属二院神经内科,215004 [2]苏州医学院附属二院神经外科,215004

出　　处：《中国临床神经科学》2000年第2期89-91,共3页Chinese Journal of Clinical Neurosciences

摘　　要：目的：观察血性脑脊液对内皮细胞分泌内皮素（Ｅｎｄｏｔｈｅｌｉｎ，ＥＴ）和一氧化氮（Ｎｉｔｒｉｃ　Ｏｘｙｄｅ，ＮＯ）的影响。方法：建立脐静脉内皮细胞体外培养的方法，用放射免疫方法测定ＥＴ含量，用硝酸还原酶法测定ＮＯ的代谢产物ＮＯ_２～－／ＮＯ＿３～－（ＮＯ＿ｘ～－）含量，并观察血性脑脊液的作用。结果：不同时间血性脑脊液均使内皮细胞ＥＴ分泌增加，ＮＯ分泌减少。结论：血性脑脊液可使体外培养的内皮细胞ＥＴ分泌增加，ＮＯ分泌减少。Aim: To study the effect of bloody cerebrospinal fluid (BCSF) on the secretion of endothelin (ET) and nitric oxide (NO) from human endothelial cells. Methods: The endothelial cells cultures from human umbilical vein were estab- lished. Concentration of ET and metabolic production of NO,NO_2^-/NO_3^-(NO_x^-) were determined by using radioimmunoassay and HNO3 reductase respectively, and effects of bloody cerebrospinal fluid (BCSF) were conducted. Results: BCSF of various stage could induce increase of ET and decrease of NO_x^- (P<0. 01) secreted by endothelial cells (P<0. 01). Conclusion: BSCF could induce increase of ET and decrease of NO_x^- secreted by endothelial cells cultured.

关 键 词：蛛网膜下腔出血 血性脑脊液 内皮素 一氧化氮 

分 类 号：R743.350.2[医药卫生—神经病学与精神病学] R446.14[医药卫生—临床医学]