1株二甲四氯降解菌的分离鉴定及其降解特性  

Isolation,identification and degradation characteristics of a MCPA-degrading bacterial strain

作　　者：江紫薇[1] 谭济才[1] 谭琳[1] 曾维爱[1,2] 李宏光 蒋瑒[1] 

机构地区：[1]湖南农业大学生物安全科学技术学院,湖南长沙410128 [2]湖南省烟草公司郴州市公司,湖南郴州423000

出　　处：《湖南农业大学学报（自然科学版）》2012年第6期642-647,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省烟草公司项目(10–12Aa02);上海烟草集团项目(SZBCW2011)

摘　　要：采用富集培养法,从湖南省郴州烟区土壤中分离得到1株可降解二甲四氯的细菌SE08,经过形态、生理生化特征及16S rDNA序列分析,初步鉴定为肠杆菌属(Enterobacter sp.)。SE08对二甲四氯的降解特性研究表明,SE08能有效降解质量浓度为150～1 000 mg/L的二甲四氯,在30℃条件下,24 h内对500 mg/L二甲四氯的降解率达到45.21%。菌株SE08降解二甲四氯的最适pH值为6.0,最适温度为30℃。A bacterial strain named SE08 capable of degrading 2-methyl-4-chlorophenoxy acetic acid （MCPA） was newly isolated by enrichment culture from the tobacco field soil in Chenzhou district, Hunan province. Strain SE08 was temporarily identified as Enterobacter sp. based on morphological and physio-biochemical characteristics combined with 16S rDNA sequence analysis. Strain SE08 could effectively degrade MCPA in concentration range 150-1 000 mg/L. And 45.21% of MCPA （500 mg/L） could be degraded by strain SE08 in 24 h. The optimal pH value and temperature for MCPA degradation by SE08 was 6.0 and 30 ℃, respectively.

关 键 词：二甲四氯 降解 肠杆菌属 

分 类 号：X172[环境科学与工程—环境科学]