实验性癫痫大鼠海马损害与磁共振波谱对照研究  

The Comparative Study of MRS and Hipocampal Damage in Epilepsy Model of Rat

作　　者：叶静[1] 张文波[2] 祁吉[2] 韩悦[3] 廉宗澄[3] 孙平川[4] 黎明[4] 

机构地区：[1]华西医科大学附属第一医院精神科,四川成都610041 [2]天津医科大学第一中心医院放射科 [3]天津医科大学第二医院MRI室 [4]南开大学吸附高分子研究所

出　　处：《实用放射学杂志》2000年第6期323-326,共4页Journal of Practical Radiology

基　　金：国家自然科学基金资助!批准号39700037

摘　　要：目的 :观察癫痫持续不同时间氢质子磁共振波谱 (1 H MRS)检测的致痫灶 NAA和 Cho异常与海马结构损害的相关性。方法 :2 1只 Wistar大鼠 ,实验组 15只 ,10 % Pilocarpine 35 0 mg/ kg腹腔注射 ,诱发反复全面强直阵挛发作 ,光镜观察发作持续 5min至 6 0 h大鼠海马区神经元和胶质细胞变化 ,并计数海马 CA1 和 CA3段残存正常神经元数量 ;离体1 H MRS测定代谢产物 NAA和 Cho含量变化。结果 :癫痫持续 (SE) 5～ 30 min,光镜观察到神经元变性 ,1 HMRS检测出 NAA值降低 ;SE30 min～ 3h部分神经元坏死、少许丢失 ,轻度胶质增生 ,NAA明显降低 ;SE3～ 6 h后 ,大部分神经元坏死、丢失 ,胶质增生明显 ,形成典型的海马硬化 ;除NAA值降低外 ,1 HMRS还检测出 Cho值升高。结论 :1 HMRS检出的 NAA可反映癫痫脑损害程度 ,并且癫痫发作持续时间与 NAA值和残存正常神经元数量呈显著的负相关。Cho值升高在海马硬化形成后检出 。Objective:To study the relationship between hippocamal damage and abnormalities of NAA,Cho with 1HMRS.Methods:21 Wistar rats were injected with 10% pilocarpine intraperitoneal to induce generalized tonic-clonic seizure(GTCS).Six rats were used as control.According to status epilepticus(SE),the epileptic rats were divided into 4 groups.The brain samples were got 30 μm axial slices,stain with HE,observe the changes of histology in hippocampal regions with different seizure time,and the amount of remaining neurons in CA 1 and CA 3 of hippocampus were counted.The concentrations of NAA(N-acetyla-spatate)and Cho(cholin)were determined in vitro with 1HMRS.Results:After 5 min～30 min of SE,the neuronal degeneration were found,and concentration of NAA decreased.SE 30 min～3 hours,the amount necrotic neurons increased.There were small amount of neuronal loss and gliosis;The concentration of NAA increased significantly.After 3～6 hours of SE,most of neurons were disappeared and gliosis,then the typical hippocampal sclerosis was formed.Besides of the NAA decreased,the increased concentration of Cho was determined.Conclusion:The lasting time of seizure is negative correlation to the concentration of NAA and the amount of remained normal neurons,it is sensitive marker in indicating the brain damage.The increased concentration of Cho may imply gliosis.

关 键 词：氢质子磁共振波谱 癫痫 海马硬化 大鼠 

分 类 号：R742.104[医药卫生—神经病学与精神病学] R816.1[医药卫生—临床医学]