分光光度法测定硒盐中的硒(Ⅳ)含量  

Determination of Selenium(Ⅳ) Content in Salt by UV-Vis Spectrophotometry

作　　者：陈爱敏[1] 魏海英[1] 

机构地区：[1]河北大学化学与环境科学学院,河北保定071002

出　　处：《中国无机分析化学》2012年第4期70-73,共4页Chinese Journal of Inorganic Analytical Chemistry

基　　金：河北省科技厅科技计划项目(11276435)

摘　　要：室温下硒(Ⅳ)与邻苯二胺配位生成黄色配合物,用环己烷萃取后,在最大吸收波长330nm处测定其吸光度。实验结果表明,硒含量在4～20μg/mL时线性关系良好,线性方程A=0.1999c-0.0011(R=0.9995)。最优化条件下测定硒盐中硒的含量取得满意结果。方法操作简单,设备和药品价格低廉,准确度高,稳定性好,适宜于基层实验室对食品中微量元素硒的检验。Selenium（Ⅳ） can coordinate with o-phenylenediamine and form a yellow complex at room tem- perature. The absorbance of the complex after being extracted using cyclohexane was measured at its maxi- mum absorption wavelength of 330nm using a UV-Vis spectrophotometer. The experimental results shows that there is a good linear relationship between the UV absorption and the Se（Ⅳ） concentration in the range of 4-20μg/mL. The regression equation was A=0. 1999c=0. 0011（R=0. 9995）. The content of Se （Ⅳ） in salt was determined under the optimum condition and the results was satisfied. Since this method is simple and has low cost for instrument and reagent usage, high accuracy and good stability, so it is suit- able to be used by industrial laboratories for determination of trace Se（Ⅳ） in food product.

关 键 词：紫外分光光度法 硒 硒盐 

分 类 号：O657.32[理学—分析化学] TH744.122[理学—化学]