2011年南京市外环境水体及水产品致病性弧菌污染状况分析  

Analysis on Polluting Situation of Causative Vibrio in External Environmental Water and Sea Products in Nanjing Area from 2011

作　　者：花蕾[1] 程婷婷[1] 

机构地区：[1]南京市疾病预防控制中心,江苏南京210003

出　　处：《中国医学创新》2012年第34期3-5,共3页Medical Innovation of China

摘　　要：目的:了解南京市外环境水体与水产品中致病性弧菌污染状况,预防和控制此类食物中毒的发生。方法:采集外环境水体与水产品,用碱性蛋白胨水增菌、庆大琼脂、TCBS琼脂、科玛嘉弧菌显色琼脂分离,对分离出的致病性弧菌进行系统生化鉴定,对分离出的霍乱弧菌用血清学和多重荧光PCR方法进行分型与毒力检测。结果:2011年共采集南京市外环境水体与水产品共151份,其中检出致病性弧菌8种,共38株,检出率依次为霍乱弧菌7.28%、副溶血弧菌5.83%、嗜水气单胞菌4.64%、溶澡弧菌3.33%、温和气单胞菌3.31%、创伤弧菌1.67%、梅氏弧菌0.83%、类志贺邻单胞菌0.83%。其中分离出的霍乱弧菌均为非01/非0139群霍乱弧菌且不携带霍乱肠毒素。结论:2011年水产品中非01/非0139群霍乱弧菌污染较为严重,其次为副溶血弧菌、创伤弧菌等;外环境水体中嗜水气单胞菌污染较为严重,需加强外环境水体与水产品的监测,做好预防控制疾病工作。Objective：To understand contamination status of external environmental water and sea products in Nanjing, prevention and treatment of food poisoning.Method：External environmental water and sea products samples were collected, samples were proliferated in alkaline liquid mediam, and purified in Gentamyein Agar, Thiosulfate-Citrate-Bile-Suerose agar and chromager vibrio colored agar, The virulence gene and phenotype of the isolated strains were detected by coagulation with diagnostic serum and multi-fluorescent PCR.Result ： 2011 In Nanjing, collection a total of 151 environmental water and sea products, with 38 strains of eight kinds of causative vibrio isolated, the rates of isolation of V.chelera, V.parahaemolyticus, Aeromonas hydrophila, V.alginalytieus, Aeromonas mild, V.vulnicus, V.metschnikovi, Plesiomonas shigelloides were 7.28%, 5.83%, 4.64%, 3.33%, 3.31%, 1.67%, 0.83% and 0.83%, respecvtively.The vibrio cholera were Non-01 Non-0139 vlbrio cholera and not carry cholera toxin.Conclusion ： In 2011, sea products from Non-01 Non-0139 vibrio cholera polluted more serious, Secondly, Vibrio parahaemolytieus, Vibfio vulnifleus etc, in external water, aeromonas hydrophila relatively serious, must to strengthen the monitoring and disease prevention of environmental water and aquatic products.

关 键 词：致病性弧菌 外环境水体 水产品 霍乱肠毒素 

分 类 号：R123[医药卫生—环境卫生学]