微小核糖核酸-34a在膀胱癌组织中表达的临床意义  

Expression and role of miR-34a in bladder cancer

作　　者：王伟[1] 李覃[2] 石理华[1] 李瑛[1] 李辉[1] 

机构地区：[1]武警后勤学院附属医院泌尿外科,天津300162 [2]武警后勤学院免疫学教研室

出　　处：《中华泌尿外科杂志》2012年第12期939-942,共4页Chinese Journal of Urology

基　　金：天津市应用基础与前沿技术研究计划（11JCYBJC14600）;武警后勤学院附属医院种子基金面上项目（FYM201211）

摘　　要：目的探讨微小核糖核酸-34a（miR-34a）在膀胱癌组织中表达的临床意义。方法膀胱癌手术切除标本42例，按照UICC2002TNM临床分期系统，非肌层浸润性癌17例，肌层浸润性癌25例；按照WHO1973分级系统，低级别18例，高级别24例。癌旁5cm正常膀胱黏膜标本42例作为对照组。采用荧光实时定量PCR法检测组织中miR-34a的基因表达水平。应用化学方法合成成熟miR-34a瞬时转染膀胱癌T24细胞，流式细胞术检测miR-34a对T24细胞凋亡和生长增殖的影响。结果42例膀胱癌组织标本中miR-34a显著低表达26例（61．9％），不表达11例（26．5％），高表达5例（11．6％），且miR-34a的低表达与肿瘤大小及恶性程度相关，肿瘤越大，恶性程度越高，miR-34a表达量越低。转染miR-34a模拟物后T24细胞生长增殖明显受抑，细胞凋亡率为（9．11±0．41）％，与阴性对照组（3．05±0．29）％和空白对照组（3．73±0．55）％相比差异有统计学意义（P〈0．01）。结论低表达miR-34a可能是膀胱癌组织重要的生物学特征，并参与膀胱癌的生物学进程。Objective To investigate the expression and biological function of microRNA-34a （miR-34a） in bladder cancer. Methods Forty-two cases of bladder cancer specimen, including 17 non- invasive carcinoma and 25 muscle invasive carcinoma classified by UICC 2002 TNM, or 18 low-grade and 24 high-grade classified by WHO 1973. Meanwhile, mucosa adjacent to the carcinoma was selected as normal control. The gene expression of miR-34a was determined using real-time quantitative polymerase chain reac- tion in 42 cases of bladder carcinoma samples. Mature mimics of miR-34a were chemically synthesized and transiently transfected into T24 bladder cancer cells. The effects of miR-34a on apoptosis and proliferation in T24 cells were evaluated by flow cytometry and MTS respectively. Results 61.9% of carcinoma samples showed low expression of miR-34a, which was correlated with the malignancy and tumor size of bladder car- cinoma. 26.5% （11 cases） was negative and 11.6% （5 cases） showed high expression. Furthermore, up- regulation of miR-34a in T24 cells contributed to cell growth and apoptosis. The apoptosis rate of T24 cells was （9.11 ±0.41 ）% , which had significant difference compared with NC mimics and blank control group respectively （P 〈 0. O1 ）. Conclusion The relative low expression of miRNA-34a may be involved in the tumorigenesis and development of bladder carcinoma.

关 键 词：MIR-34A 膀胱肿瘤 癌 凋亡 

分 类 号：R737.14[医药卫生—肿瘤]