经上丘逆行标记法视网膜神经节细胞定量的实验研究  

An experimental study on quantitative retrograde labeling of retinal ganglion cells of normal rats via superior colliculus

作　　者：朱益华[1] 蒋幼芹[1] 刘忠浩[2] 罗学港[2] 吴振中[1] 

机构地区：[1]湖南医科大学第二附属医院眼科,长沙410011 [2]湖南医科大学神经生物学研究室

出　　处：《眼科研究》2000年第3期218-220,共3页Chinese Ophthalmic Research

摘　　要：目的　建立一种视网膜神经节细胞 (RGCs)定性及定量的研究方法 ,并了解大鼠RGCs密度分布情况。方法　暴露大鼠双上丘后将饱含 3 0 %辣根过氧化物酶 (HRP)溶液的明胶海绵片贴附于双上丘表面 ,大鼠存活 2 4h后 ,取出视网膜行HRP组织化学反应 ,计算机图像分析系统对RGCs进行分类及计数。结果　左右眼RGCs密度分别为 14 66±3 76/mm2 及 14 5 7± 40 4/mm2 ,左眼鼻、颞侧RGCs密度分别为 14 90± 3 71/mm2 及 14 4 2± 3 82 /mm2 ,右眼鼻、颞侧RGCs密度分别为 14 67± 410 /mm2 及 14 4 6± 40 0 /mm2 。结论　成功地建立了直接上丘HRP逆行标记RGCs定量计数法 ,正常大鼠双眼RGCs密度基本相同 ,颞侧及鼻侧RGCs密度基本相同。ObjectiveTo establish a quantitative method that might help to reveal the distribution of retinal ganglion cell(RGC)density in the rat.MethodsA skin incision is made along the sagittal suture to expose the skull and bilateral with 0 5cm×5cm holes was made in parietal bone using a dental drill.The dura mater is partially removed covering the superior colliculus(SC) removed by electric suction.By this process,bilateral SC were totally exposed.A small piece of gelfoam sheet fully sucked on the surface of two SCs.After 24?h,the retina was peeled off and HRP histochemistry reaction was performed.Counting and classification of RGCs were made by computerized image analyzer.ResultsMean±SD of the RGCs density from the left eye and from the right eye was 1?466±376/mm 2 and 1?457±404/mm 2 respectively.Mean±SD of the RGCs density from nasal and temporal retina in left eye was 1?490±371/mm 2 and 1?442±382/mm 2,whereas that of in right eye was 1?467±410/mm 2and 1?446±400/mm 2.Different parts of retina have different RGCs density,mean±SD of the RGCs density in the right eye were 1?720±395/mm 2(center), 1?438±336/mm 2(middle) and 1?212±269/mm 2(periphery),whereas that of in left eye were 1?680±366/mm 2(center),1?466±330/mm 2(middle)and 1?253±304/mm 2(periphery).The constituent ratio of large,middle and small RGCs in normal rats were 22%,25% and 53%, respectively.ConclusionQuantitative retragrade labeling by axoplasmic transport of RGCs of normal rat via SC was established successfully.The RGC density didn’t vary from bilateral eyes and from temporal and nasal retina in normal rats has no significant difference,but in the center,middle and peripheral part of the retina RGCs density were different significantly.Small RGCs occupy the major percentage in the retinal

关 键 词：视网膜神经节细胞 上丘 辣根过氧化物酶 

分 类 号：R775[医药卫生—眼科]