体外培养牛眼小梁细胞表达转化生长因子β及其受体蛋白  

Expression of TGF-β and TGF-β receptors protein in cultured bovine trabecular meshwork cells

作　　者：曹阳[1] 魏厚仁[1] 富名水[1] 麦才铿[1] 丁正平[1] 吕源淑[1] 张缨[1] 

机构地区：[1]同济医科大学附属协和医院眼科,武汉430022

出　　处：《眼科研究》2000年第3期235-237,共3页Chinese Ophthalmic Research

摘　　要：目的　从蛋白质水平了解体外培养牛眼小梁细胞是否表达转化生长因子 β(TGF β)及其受体 (TGF βR)。 方法　采用Supervision法对体外培养第 3代牛眼小梁细胞进行TGF β1,TGF βR免疫组化检测。 结果　小梁细胞TGF β ,-β2 和TGF βRⅠ ,RⅡ ,RⅢ免疫组化染色的阳性信号分别位于胞浆内和胞膜上 ,阴性对照未见阳性信号。 结论　牛眼小梁细胞表达TGF β ,TGF βR蛋白。较之猪眼小梁细胞 ,牛眼小梁细胞更适用于今后研究TGF β及其受体与原发开角型青光眼发病学的关系。ObjectiveTo study whether cultured bovine trabecular meshwork cells express transforming growth factor β(TGF β) and TGF β receptors(TGF βR) in protein level.MethodsBovine trabecular meshwork cells were cultured in vitro and passaged 3 times,then the cells were transfered or cultured onto sterile cover lips,and the expressions of TGF β and TGF βR were detected with immunohistochemical technique Supervision method.ResultsTGF β 1, β 2were significantly expressed in the cytoplasm of cultured bovine trabecular meshwork cells,and TGF βR Ⅰ,RⅡ,RⅢ immunostain were positive on the membrane of the cells.No immunostaining was seen in the negative control.ConclusionThe presence of TGF β and TGF βR in cultured bovine trabecular cells were confirmed.Bovine trabecular cells may be more suitable to investigate the relationship between TGF β,TGF βR and primary open angle glaucoma than porcine trabecular cells.

关 键 词：小梁网 细胞培养 TGF-βR 开角型青光眼 

分 类 号：R775[医药卫生—眼科]