正畸牙齿移动刺激信息传导通路的动物实验研究  

A Study of Sensory Pathway in Central Nervous System ;of Experimental Tooth Movement in Rats

作　　者：冯雪[1] 王凌[2] 林珠[1] 段银钟[1] 

机构地区：[1]第四军医大学秦都口腔医院正畸科,710032 [2]第四军医大学神经生物研究所

出　　处：《华西口腔医学杂志》2000年第3期153-155,共3页West China Journal of Stomatology

摘　　要：目的 :研究丘脑腹后内侧核 (VPM )是否接受来自三叉神经脊束核尾侧亚核 (Vc)传入的牙齿移动性刺激信息 ,从而探讨牙齿移动引起的伤害性刺激信息在中枢神经系统内的感觉传导通路。方法 :用微量注射器将 2 %荧光金 (FG)注入大鼠VPM 4～ 6d后 ,进行对侧的实验性牙齿移动 2h ,用FOS蛋白免疫组化方法观察Vc内神经元对c fos的表达以及是否存在FG与FOS双重阳性的神经元。结果 :FOS阳性神经元密集分布于同侧Vc浅层 ,呈带状 ,背外侧居多 ;FG逆行标记的神经元主要见于对侧三叉神经感觉核簇的脊束核及感觉主核 ,Vc的全长均有FG标记细胞 ,主要分布于Ⅰ层、Ⅰ Ⅱ层交界处。在Vc各层内均有FG与FOS的双标记细胞 ,约占Vc内FG逆行标记细胞总数的 5%。结论 :牙齿移动性刺激信息在Vc内中继 ,Vc向VPM有投射神经元 。Objective: To investigate whether ventral posteromedial thalamus nucleus (VPM) receive the stimulation from experimental tooth movement in rats through the conduction of spinal trigeminal caudal nucleus (Vc), thus to study the central sensory pathway of tooth movement. Methods: 2% fluorogold (FG) was injected into rat's VPM using microinjector through retrograde tracing technique. After 4～6 days, the experimental tooth movement was conducted in contra side against FG injection. 2 hours later, animals were sacrificed and FOS protein immunocytochemical reaction was conducted. The sections were observed under Olympus fluorescence microscope. Results: FOS immunocytochemical positive neurons were densely distributed in epilateral superfacial layer of Vc, being belt like with more in dorsolateral 2 hours after experimental tooth movement; FG labeled neurons were found in Vc and principle trigeminal nucleus complex, especially within the whole length of Vc, with more in layer Ⅰ and the junction of layers Ⅰ and Ⅱ. There were both FOS immunocytochemically positive and FG labeled neurons, approximately 5% of the whole FG labeled neurons. Conclusion: As a special oro facial stimulation, there are corresponding parts in the central nervous system according to tooth movement. The stimulating message intergrated in Vc and Vc has projective neurons to VPM, and VPM might be a relay station of the stimulating message of experimental tooth movement in rats. ;

关 键 词：丘脑腹后内侧核 VC 牙齿移动 FOS蛋白 正畸 

分 类 号：R-331[医药卫生]