聚季铵盐-1的抑菌活性及其稳定性研究  

Study on bacteriostatic activity and stability of polyquaternium-1

作　　者：贾瑞[1,2] 贺艳丽[1,2] 

机构地区：[1]山东大学药学院,山东济南250012 [2]山东省生物药物研究院博士后科研工作站,山东济南250101

出　　处：《中国生化药物杂志》2012年第6期782-785,共4页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的研究聚季铵盐-1的抑菌活性及其稳定性。方法采用营养肉汤试管二倍稀释法测定聚季铵盐-1对大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌、白色念珠菌和黑曲霉的最低抑菌浓度(MIC)和最低杀菌浓度(MBC),并研究紫外光照射、温度、pH和盐浓度对聚季铵盐-1稳定性的影响。结果聚季铵盐-1对5种试验菌的MIC依次为16,4,16,8,64μg/mL;对5种试验菌的MBC依次为16,8,32,8,128μg/mL。聚季铵盐-1的抑菌活性不受紫外光和pH的影响,但会随温度和盐浓度的升高而降低。结论聚季铵盐-1在较低浓度具有抑制微生物生长的活性,对紫外照射和pH较稳定,高温易使其分解,在高渗环境下,其抑菌活性也会显著降低。Purpose To study the bacteriostatic activity and stability of polyquaternium-1 （PQ-1） Methods The minimal inhibitory concentration（MIC） and minimal bactericidal concentration （MBC） of PQ-1 to Escherichia coli , Staphyloccocus aureus , Pseudomonas aeruginosa , Candida albicans and Aspergillus niger were determined through double broth dilution method. The effects of UV irradiation, temperature, pH and salt concentration on stability of PQ-1 were also evaluated. Results The MIC of PQ-1 to the test microbes were 16,4,16,8 and 64 μg/mL respectively, and the MBC were 16,8,32,8 and 128 μg/mL re- spectively. The bacteriostatic activity of PQ-1 was not affected by UV irradiation and pH,but decreased with the rise of temperature and salt concentration. Conclusion PQ-1 with low concentration displayed the strong bacteriostatic activities. And it was relatively stable to UV irradiation and pH. However,it was heat- sensitive and easily deactivated in hyperosmotic salt solution.

关 键 词：聚季铵盐-1 抑菌活性 最低抑菌浓度 稳定性 

分 类 号：R962[医药卫生—药理学] R927.11[医药卫生—药学]