响应面法优化番石榴多糖的超声提取工艺  

Optimization of ultrasound-assisted extract polysaccharides from Psidium guajaua L.by response surface methodology

作　　者：蒋顶云[1] 蒋长兴[1] 熊清平[1] 戴守庆[1] 李晨亮[1] 董彤彤[1] 

机构地区：[1]淮阴工学院生命科学与化学工程学院,淮安223003

出　　处：《食品科技》2012年第12期174-178,共5页Food Science and Technology

基　　金：江苏省自然科学基金项目(SBK200930185);江苏省淮安市工业基金项目(HAG0706)

摘　　要：目的:研究筛选番石榴多糖的超声最佳提取工艺条件。方法:选定提取温度、时间及水料比作为影响因素,以番石榴多糖提取率为评价指标。在单因素试验的基础上,通过3因素3水平Box-Behnken中心组合试验,建立多糖提取率的二次多项式回归方程,经响应面回归分析得到优化组合条件。结果:最佳提取工艺条件为提取温度73℃、超声时间21min、水料比27:1(v:m,mL/g),在该条件下多糖提取率预测值为85.73%,验证值为(85.54±1.04)%(n=3)。结论:此方法与传统水提取法相比具有省时、高效的优点,为番石榴多糖的后续研发提供试验基础。Objective： To optimize technology of ultrasound-assisted extract polysaccharides from Psidium guajaua L, by response surface analysis. Methods： On the basis of single-factor experiments, the relationship between main extraction conditions including extraction time, the ratio of water to raw material and temperature was modeled using a 3-factor, 3-level Box-Behnken experimental design and studied the yield of polysaccharides. And then, the established model was analyzed by response surface methodology to obtain the optimum extraction conditions. Results： The optimum extraction conditions were obtained as follows： extraction time was 21 min, the ratio of water to raw material was 27：1（v：m, mL/ g） and temperature was 73 ℃. Under these conditions, the estimated and observed values of maximal yield of polysaccharides were 85.73% and （85.54±1.04）% （n=3）, respectively. Conclusion： The method has the advantage of time-saving and efficient compared with the traditional water extraction, providing the experimental basis for the follow-up research and development of Psidium polysaccharides.

关 键 词：番石榴 多糖 超声辅助提取 响应面分析法 

分 类 号：R284.2[医药卫生—中药学]