玉米芯发酵产黄原胶及醇析工艺优化研究  

Utilization of corncob for the production of xamthan gum and optimized the conditions of alcohols precipitation

作　　者：何海燕[1] 覃拥灵[1] 唐媛媛[1] 梁艳华[1] 

机构地区：[1]河池学院化学与生命科学系,宜州546300

出　　处：《食品科技》2012年第12期256-258,263,共4页Food Science and Technology

基　　金：广西自然科学基金项目(2010GXNSFA013103)

摘　　要：实验利用玉米芯为碳源发酵生产黄原胶,通过正交设计确定优化的发酵条件为:发酵培养基蛋白胨浓度0.3%、柠檬酸含量0.01%、发酵液初始pH7、发酵温度33℃、发酵时间为96h,在优化的发酵条件下黄原胶的产量可达20.78g/L;实验还对黄原胶醇析工艺进行了优化研究,确定了发酵液添加4%硝酸钾、3倍发酵液体积95%乙醇的最佳醇析工艺。Xamthan gum was produced by corncob, after the orthogonal design experiments, the optimal combinations were confirmed as follow： The content of peptone was 0.3%, the citric acid content was 0.01%, initial pH of culture medium was 7, and then cultured on the shaking table at 33 ℃ for about 96 h, under the optimized conditions, the xamthan gum content was 20.78 g/L; The paper also optimized the conditions of alcohols precipitation, concluded that when the fermentation broth add 4% Potassium nitrate, 95% ethanol that three times the fermentation broth volume, the yield of xamthan gum was highest.

关 键 词：黄原胶 玉米芯 正交设计 醇析 野油菜黄单胞菌 

分 类 号：TS202.3[轻工技术与工程—食品科学]