不同浓度吗啡对5-氟尿嘧啶诱导的MCF-7乳腺癌细胞凋亡时Bcl-2、Bax表达的影响  

Effects of different concentrations of morphine on Bcl-2 and Bax expressions in the process of MCF-7 cellular apoptosis induced by 5-Fluorouracil

作　　者：郝振军 王志学[2] 孙立新[3] 闫彩云[2] 李汝泓[2] 雷燕[4] 

机构地区：[1]承德市妇幼保健院麻醉科,河北承德067000 [2]承德医学院附属医院麻醉科 [3]承德医学院附属医院中心实验室 [4]承德医学院附属医院手术室

出　　处：《中国老年学杂志》2012年第23期5164-5166,共3页Chinese Journal of Gerontology

基　　金：承德市科学技术研究与发展计划项目(No.201121037)

摘　　要：目的研究吗啡与5-氟尿嘧啶(5-Fu)联合应用对MCF-7乳腺癌细胞Bcl-2、Bax表达的影响。方法药物作用48 h后通过免疫细胞化学技术检测Bcl-2、Bax表达情况。结果 Bcl-2表达情况:未处理组最强。单独应用吗啡处理时,浓度为250、1 250μmol/L引起Bcl-2表达减弱(P<0.05,P<0.01),三种浓度的吗啡与5-Fu联合应用均具有协同作用(P<0.05)。Bax表达情况:未处理组最弱。单独应用吗啡处理时,250、1 250μmol/L浓度的吗啡引起Bax表达增强(P<0.05,P<0.01),三种浓度的吗啡与5-Fu联合应用,均具有协同作用(P<0.05)。结论一定浓度的吗啡能与5-Fu发挥协同作用,强化5-Fu诱导的Bcl-2表达下调、Bax表达上调,且与吗啡的浓度有一定的剂量依赖关系。Objective To study on the effects of different concentrations of morphine on Bcl-2 and Bax expressions in the process of MCF-7 cellular apoptosis induced by 5-Fluorouracil. Methods After treated for 48 h, the expressions of Bcl-2 and Bax were detected by immunocytochemistry. Results The expression of Bcl-2 of untreated group was the strongest. Using 500 p.mol/L 5-FU only, the expression of Bcl-2 was decreased （P 〈 0. 01 ）. Using morphine only, 250 and 1 250 μmol/L of morphine leaded to decreasing the expression of Bcl-2 （P 〈 0. 05 ,P 〈 0. 01 ）. Using morphine combined with 5-FU, the synergisms of morphine with 5-FU were found in all three groups （P 〈 0. 05 ）. The expression of Bax in untreated group was the weakest. Using 500 μmol/L 5-FU only, the expression of Bax was increased（P 〈 0. 01 ）. Using morphine only, 250 and 1 250 μmol/L of morphine leaded to increasing the expression of Bax （P 〈0. 05 ,P 〈0. 01 ）. Using morphine combined with 5-FU, the synergisms were found in all three groups （P 〈 0. 05）. Conclusions Morphine in certain concentration could enhance significantly the down-regulating effect on Bcl-2 expression and up-regulating effect on Bax expression that are induced in MCF-7 breast cancer cells, in certain dose dependent manner. Combination of morphine and 5-FUcould show synergisms

关 键 词：吗啡 5-氟尿嘧啶 MCF-7 BCL-2 BAX 

分 类 号：R73-36[医药卫生—肿瘤] R34[医药卫生—临床医学]