蛋白水解诱导因子在消化系统肿瘤癌性恶液质患者中的表达及其作用  

Proteolysis induce factor in the digestive systematic cancer cachexia patients： its expression and role in cancer cachexia

作　　者：韩寓嵩[1] 庄秋林[1] 吴国豪[1] 

机构地区：[1]复旦大学附属中山医院普通外科,上海200032

出　　处：《中华胃肠外科杂志》2012年第12期1287-1290,共4页Chinese Journal of Gastrointestinal Surgery

摘　　要：目的观察蛋白水解诱导因子（PIF）在消化系统肿瘤癌性恶液质（CC）患者中的表达情况。探讨其在消化系统肿瘤CC发生发展中的作用。方法检测28例消化系统肿瘤CC患者、13例消化系统肿瘤非CC患者和12例非肿瘤消瘦患者尿液中PIF的表达。将PIF作用于离体小鼠C2C12细胞．测定细胞蛋白激酶B（Akt）磷酸化程度并观察细胞形态。结果CC患者PIF阳性率为53．6％（15／28），其他两组均无PIF阳性表达。4nmol／L的PIF蛋白作用于C2C12细胞0．5h后，细胞磷酸化Akt的表达量增高，并且细胞呈现不同程度的萎缩、变形或死亡。结论PIF特异性高表达于消化系统肿瘤CC患者的尿液中．PIF可能通过激活Akt的磷酸化从而引发下游的蛋白水解途径导致消化系统肿瘤CC。Objective To demonstrate the expression of proteolysis induce factor （PIF） in the gastrointestinal （GI） cancer cachexia patients and evaluate its role in cancer cachexia. Methods Examination of PIF was performed in urine samples from 28 GI cancer cachexia patients, 13 GI cancer patients without cachexia, and 12 weight loss patients with benign disease. PIF was added to the mice cultured C2C12 muscle cells, then the protein kinase B （Akt） phosphorylation and morphological change were measured. Results The positive rate of PIF in urine of 28 cancer cachexia patients was 53.6% （15/28）. In the other two groups, no positive result was detected. PIF could successfully induce Akt phosphorylation, cell atrophy, metamorphosis, and death. The peak of this phosphorylation could be detected after half an hour of the initiation of PIF at a concentration of 4 nmol/L. Conclusions PIF is specifically and highly expressed in GI cancer cachexia patients＇urine. PIF can induce cancer cachexia possibly by activating Akt phosphorylation and inducing downstream proteolysis.

关 键 词：消化系统肿瘤 癌性恶液质 蛋白水解诱导因子 蛋白激酶B 磷酸化 

分 类 号：R735[医药卫生—肿瘤]