复方丹参片对阿尔茨海默病转基因细胞模型Aβ表达的影响  

Effects of Compound Danshen Tablet on the Expression of Aβ in Transgenic Cell Model of Alzheimer′s Disease

作　　者：胡华[1] 周德生[1] 喻嵘[1] 孙晓鹏[1] 覃仁安[2] 杨洋[1] 王佳君[1] 

机构地区：[1]湖南中医药大学第一附属医院神经内科,长沙410007 [2]广州白云山和记黄埔中药有限公司现代中药研究院,广州510515

出　　处：《中国中西医结合杂志》2012年第12期1663-1666,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金："重大新药创制"国家科技重大专项(No.2011ZX09201-201-01)

摘　　要：目的研究复方丹参片对阿尔茨海默病(AD)转基因细胞模型β-淀粉样肽(Aβ)表达的影响。方法用前期试验已构建成功的转基因细胞模型做为AD转基因细胞模型,0.5、1、2、4倍复方丹参片饲养大鼠,取0.5、1、2、4倍浓度大鼠血清培养AD转基因细胞,并在24、48、72h用Western blot方法检测转基因细胞Aβ的表达情况。结果 24h,1倍剂量组量效关系最佳。结论复方丹参片能减少AD转基因细胞模型Aβ的表达。Objective To study the effects of Compound Danshen Tablet （CDT） on the expression of the β-amyloid peptide （Aβ） in transgenic cell model of Alzheimer＇s disease （AD）. Methods The previously successfully cultured transgenic cell model was used as AD transgenic cell model. Rats were fed with 0.5, 1,2, and 4 times CDT. The AD transgenic cells were cultured in 0.5, 1,2, and 4 times rats＇ sera. The expressions of Aβ in transgenic cells were detected using Western blot. Results The optimal dose effect was obtained in the 1 time CDT group at 24 h. Conclusion CDT could reduce the Aβ expression level in the AD transgenic cell model.

关 键 词：复方丹参片 阿尔茨海默病 Β-淀粉样肽 AD转基因细胞模型 

分 类 号：R749.16[医药卫生—神经病学与精神病学]