血浆hsa-miR-150和hsa-miR-375在结直肠癌中的表达及意义  

The expressions of plasma hsa-miR-150 and hsa-miR-375 in patients with colorectal cancer and its clinical significance

作　　者：徐伶玲[1] 马莹[2] 张文娟[1] 安广宇[1] 吴静[1] 

机构地区：[1]首都医科大学附属北京世纪坛医院消化内科,北京100038 [2]西安交通大学医学院临床医学系,陕西西安710061

出　　处：《肿瘤》2012年第12期1001-1008,共8页Tumor

基　　金：国家高技术研究发展计划(863计划)(编号:2012AA02A506)

摘　　要：目的:筛选结直肠癌患者血浆微小RNA(microRNA,miRNA),并寻找与临床特征相关的miRNA。方法:通过TaqMan Human Micro RNA Array检测7例结直肠癌患者和6例健康体检者血浆中差异表达的miRNA,应用实时荧光定量PCR在29例结直肠癌患者和20例健康体检者中对TaqMan Human Micro RNA Array检测结果进行验证,分析血浆差异表达的miRNA与结直肠癌患者临床特征之间的关系。结果:在变化倍数>1.0,P<0.05的条件下初步筛选得到16个结直肠癌患者血浆差异表达miRNA,实时荧光定量PCR验证得到hsa-miR-125b、hsa-miR-375、hsa-miR-150和hsa-miR-206与TaqMan Human Micro RNA Array检测结果一致,并且在结直肠癌患者和健康体检者血浆中的表达差异有统计学意义(P<0.05)。同时发现,血浆hsa-miR-150和hsa-miR-375表达水平与肿瘤的浸润深度或脉管瘤栓有关。结论:血浆hsa-miR-150和hsa-miR-375有望成为一种非侵入性的指导结直肠肿瘤分期和进行预后评估的分子标志物。Objective： To select the plasma miRNA （microRNA） associated with the clinical features of patients with colorectal cancer. Methods： Differential expression of plasma miRNA was detected by TaqMan？ Human MicroRNA Array from 7 patients with colorectal cancer and 6 healthy volunteers. The results of TaqMan？ Human MicroRNA Array were validated by using plasma samples from 29 patients with colorectal cancer and 20 healthy volunteers by real-time fluorescence quantitative-PCR, and the relationship of differential expression with clinical features was analyzed. Results： In the condition of fold change 1.0 and P 0.05, sixteen plasma-specific miRNAs were identified from patients with colorectal cancer. Hsa-miR-125b, hsa-miR-375, hsa-miR-150 and hsa-miR-206 validated by real-time fluorescence quantitative-PCR were consistent with those detected by TaqMan？ Human MicroRNA Array, and the differential expression was statistically significant between colorectal cancer patients and healthy volunteers （P 0.05）. Furthermore, the expression of plasma hsa-miR-150 and hsa-miR-375 had a significant impact on tumor invasion and vascular cancer embolus. Conclusion： Plasma hsa-miR-150 and hsa-miR-375 are expected to be non-invasive biomarkers to give assistance in tumor staging and to evaluate the prognosis of colorectal cancer.

关 键 词：结直肠肿瘤 微RNA 血浆 肿瘤标志物 

分 类 号：R735.3[医药卫生—肿瘤]