裸鼠动物模型中胰腺癌神经侵袭的研究  

A Study of Neural Invasion of Orthotopic Pancreatic Cancer in a Nude Mouse Model

作　　者：郭仁德[1] 顾建华[1] 王毅[1] 谷川[1] 

机构地区：[1]天津市第一中心医院,天津市300192

出　　处：《中国肿瘤临床》2012年第22期1769-1772,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金(编号:81172103);天津市卫生局科技基金(编号:2010KR04)资助~~

摘　　要：目的:探讨在裸鼠动物模型中胰腺癌神经侵袭发生情况。方法:人MiaPaCa-2和Capan-2胰腺癌细胞株培养,裸鼠皮下胰腺癌细胞注射成瘤后,将瘤块取出切碎后裸鼠胰腺原位移植建立裸鼠原位胰腺癌动物模型,于术后4、6、8周分批处死裸鼠,采用大体标本观察和病理切片HE染色的方法观察模型中肿瘤大小、转移情况和胰腺癌神经侵袭的情况。结果:裸鼠胰腺原位植瘤术后成瘤率100%,术后4周Mia PaCa-2组3例(50.00%)发生腹膜后神经侵犯,Capan-2组2例(33.33%)发生腹膜后神经侵犯;原位植瘤术后6周,Mia PaCa-2组5例(83.33%)发生腹膜后神经侵犯,Capan-2组4例(66.67%)发生腹膜后神经侵犯;原位植瘤术后8周,Mia PaCa-2组8例(100.00%)发生腹膜后神经侵犯,Capan-2组7例(100.00%)发生腹膜后神经侵犯。腹膜后神经侵犯组与其他组比较差异均有统计学意义(P<0.01)。结论:神经侵袭是胰腺癌发展过程中的一个早期、独立的事件,在胰腺癌发生的早期,无其他转移或很少时,已经发生了腹膜后神经侵犯。Objective：This study aims to investigate the neural invasion of orthotopic pancreatic cancer in a nude mouse model. Methods：Human pancreatic cancer cell lines MIA PaCa-2 and Capan- 2 were cultured and hypodermically injected into nude mice to set up an orthotopic animal model for pancreatic cancer. The mice were killed 4, 6, and 8 weeks post-injection, and specimens were studied using macroscopic methods. Staining with H＆E was also performed to observe tumor volume, metastasis, and neural invasion in the orthotopic xenograft animal model of pancreatic cancer. Results：The neoplasm formation ratio in the nude mice was 100%. Neu-ral invasion ratios reached 50.00% and33.33% in three cases of the MiaPaCa-2 cell line and two cases of the Capan- 2 cell line 4 weeks after orthotopic transplantation. Neural invasion ratios 6 weeks after transplantation were 83.33% and66.67% in the two groups, respec-tively. Neural ratios were 100% in both lines8 weeks after the procedure. The neural invasion rate was significantly higher in the group with orthotopic xenograft animal model than in the group with metastasis at other sites （P〈0.01）. Conclusion：Neural invasion is an ear -ly and independent event in pancreatic cancer. Pancreatic cancer has the unusual tendency of invasion into and around neural tissues, even at early stages of the disease and with or without other metastases.

关 键 词：胰腺癌 裸鼠 动物模型 神经侵袭 

分 类 号：R735.9[医药卫生—肿瘤]