产紫杉醇内生真菌Z58的分离和鉴定  

Isolation and Identification of a Taxol-producing Endophytic Fungus Z58

作　　者：苗莉云[1] 张鹏[2,3] 刘博 徐曼[2] 周蓬蓬[2] 余龙江[2] 

机构地区：[1]运城学院生命科学系,山西运城044099 [2]华中科技大学生命科学与技术学院资源生物与生物技术研究所,武汉430074 [3]湖北省农业科学研究院经济作物研究所,武汉430064

出　　处：《中国生物化学与分子生物学报》2012年第12期1141-1146,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金(No.20776058;906036);中国博士后科学基金(No.20100471183);运城学院自然科学基金(No.20060025)资助项目~~

摘　　要：本研究从曼地亚红豆杉(Taxus x media)树皮内表皮分离得到一株产紫杉醇的内生真菌Z58,通过高效液相色谱法、质谱法和核磁共振波谱法对其紫杉醇提取物进行了分析.结果表明,内生真菌Z58的紫杉醇提取物具有和紫杉醇标准品相近的色谱特征峰,其保留时间为10.2 min;也与紫杉醇标准品具有相同的质谱特征峰((M+Na)+=876)和1H-NMR谱带.并通过形态学特征分析和18S rDNA序列分析,将内生真菌Z58初步鉴定为肉座菌属(Hypocrea sp.)真菌.肉座菌Z58的紫杉醇产量约为2.5～3.0μg/g(紫杉醇/菌丝干重),是一株具有潜在应用价值的产紫杉醇内生真菌.A taxol-producing endophytic fungus was isolated from the inner bark of Taxus x media and named as Z58. The fungal extracts from Z58 were analyzed for taxol with high performance liquid chromatography （ HPLC ）, mass spectrometry （ MS ） and nuclear magnetic resonance （ NMR ） spectroscopy. The results showed that the Z58 produced taxol peaked at about the same retention time （10.2 minutes） as authentic taxol in chromatography, and with an identical signature of （M ＋Na） ＋ = 876 in mass spectrum, as well as an dentlcal H-NMR spectrum as the authentic taxol. Z58 was later i dentified to belong to Hypocrea sp. based on its morphology and conserved sequence of 18S rDNA. Hypocrea sp. Z58 was able to produce 2.5 ～3.0 μg/g （ taxol/dry weight of mycelium）. Our finding of Hypocrea sp. Z58 may be used as a promising strain for larger scale production of taxol in fermentation.

关 键 词：红豆杉 紫杉醇 内生真菌 肉座菌属 18S RDNA 

分 类 号：Q93[生物学—微生物学] R73[医药卫生—肿瘤]