胸腔积液中脱落细胞p53基因突变的检测及其意义  

Detection of p53 gene mutations and its clinical implications in the cells from pleural effusion

作　　者：万雨明[1] 曹美菊[2] 

机构地区：[1]湖北省十堰市东风公司芽箭医院,442012 [2]西安交通大学第一医院呼吸内科,西安710061

出　　处：《西安医科大学学报》2000年第3期220-222,228,共4页Journal of Xi'an Medical University(Chinese)

摘　　要：目的　探讨聚合酶链反应 -单链构象多态性分析 (PCR- SSCP)检测胸液脱落细胞 p53基因突变在恶性胸腔积液 (MPE)诊断中的可行性及价值。方法　用 PCR- SSCP分别检测了 1 9例MPE及 1 2例结核性胸腔积液中新鲜脱落细胞标本的抑癌基因— p53基因 5～ 8外显子的突变情况 ,并与正常胸膜组织进行对照。结果　 7例 MPE有 p53基因突变 ,突变率为 36.84%。其中5例发生在第 5外显子 ,2例发生在第 7外显子 ,第 6、8外显子未检出突变 ,有 2例突变检出早于临床病理诊断。结核性胸液无 1例突变。结论　提示 p53基因突变在 MPE脱落细胞中较常见 ;PCR- SSCP分析胸液脱落细胞中 p53基因突变可以作为Objective To explore the diagnostic value of detecting mutations of p53 gene in the cells from pleural effusion for malignant pleural effusion(MPE) by polymerase chain reaction single strand conformation polymor phism analysis (PCR SSCP)technique.Methods The exons 5 through 8 of p53 tumor suppressor gene of cells from 19 MPE and 12 tuberculous pleural effusion by PCR SSCP were examined.Results It was found that p53 gene mutations in 7 cases of MPE and 0 case of tuberculous pleural effusion.There were 5 cases in exon 5 and 2 cases in exon 7.No mutation was detected in exon 6 and exon 8.In the same sample, no genetic change in two or more exons was found.Conclusion The results indicate that p53 gene mutation is frequent in cells from MPE and detecting mutations of p53 gene in the cells from pleural effusion by PCR SSCP is a valuable diagnostic method for MPE.

关 键 词：P53基因 胸腔积液 脱落细胞 基因突变 

分 类 号：R561.3[医药卫生—呼吸系统]