重复经颅磁刺激对帕金森病小鼠黑质纹状体系统影响的研究  

The protective effect of repetitive transcranial magnetic stimulation on the nigrostriatal system in Parkin- son＇s disease

作　　者：王全懂[1] 顾平[2] 董巧云[1] 王彦永[1] 崔冬生[1] 王铭维[1] 

机构地区：[1]江苏连云港市第一人民医院老年医学科,连云港222002 [2]河北医科大学第一医院神经内科,河北省脑老化与认知神经科学实验室

出　　处：《中华物理医学与康复杂志》2012年第12期889-893,共5页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的观察重复经颅磁刺激（rTMS）对帕金森病（PD）模型鼠黑质纹状体系统的保护作用。方法40只雄性C57BL／6J小鼠按随机数字表法分为对照组、模型组、假磁刺激组和磁刺激组，每组10只。小鼠皮下注射1-甲基4-苯基-1，2，3，6-四氢吡啶（MPTP），按15mg／kg体重每2h注射1次，注射4次，每次间隔2h，制作帕金森病模型；用重复经颅磁刺激方法进行干预，利用免疫组化染色技术检测黑质酪氨酸羟化酶（TH）阳性神经元数目及纹状体TH阳性产物的表达变化，并借助图像分析系统对其进行定量分析；利用尼氏染色观察黑质多巴胺神经元尼氏小体（Nisslbody）的表达变化；利用嗜银染色技术显示纹状体区神经纤维的形态学变化。结果对照组、模型组、假磁刺激组和磁刺激组黑质区TH阳性神经元平均数目分别为（55．20±11．60）、（23．60±9．90）、（21．30±8．40）和（39．20±12．40），磁刺激组较对照组的明显减少（P〈0．05），但明显高于模型组和假磁刺激组（P〈0．05）；模型组和假磁刺激组比较，差异无统计学意义（P〉0．05）。对照组黑质区多巴胺神经元中布满着深蓝色呈颗粒状的尼氏体，模型组和假磁刺激组多巴胺神经元内尼氏体大量脱失，存在的尼氏体着色浅淡，磁刺激组多巴胺神经元内尼氏体有一定程度的脱失，但明显减轻。磁刺激组纹状体区TH阳性纤维校正光密度（COD）值为（0．278±0．014）较对照组的（0．304±0．012）明显减少（P〈0．05）；模型组和假磁刺激组相应的COD值分别为（0．247±0．014）和（0．243±O．030），磁刺激组分别与模型组和假磁刺激组比较，差异均有统计学意义（P〈0．05）；模型组与假磁刺激组之间比较，差异无统计学意义（P〉0．05）。对照组纹状体区可见大量呈束状、密集、排列规则有序的神经纤维；模型组和假磁�Objective To investigate the effect of repetitive transcranial magnetic stimulation （rTMS） on dopaminergic neurons in substantia nigra and nigrostriate fibers in the striatum in cases of Parkinson＇s disease （PD）. Methods Forty male C57BL/6J mice were randomly divided into a control group, a PD model group, a sham-rTMS （srTMS） group and an rTMS group with 10 mice in each group. A PD model was established by subcu- taneous injection of a solution of 1-methyl-d-phenyl-1,2,3,6-tetrahydropyridine （MPTP） , and the mice were trea- ted with rTMS or sham rTMS for 14 days. Tyrosine hydroxylase （TH） expression in the substantia and striatum were detected using an immunohistochemical technique, and the corrected optical densities （CODs） of TH in the striatum were analysed using an image analysis system. The Nissl bodies were detected by Nissl staining. The mor- phological disposition of nerve fibers in the striatum was detected using Warthin-Starry staining. Results The fraction of neurons expressing TH decreased significantly more in the substantia nigras of mice in the rTMS group than in the control group, the PD group and the srTMS group. In the control group the neural plasm of dopaminergic neurons was full of dark blue and granular Nissl bodies. Many Nissl bodies were lost in the PD and srTMS groups, and the remaining Nissl bodies were colored lightly. Few Nissl bodies were lost in the rTMS group. Positive TH reactions in the striatum were significantly decreased in the rTMS group compared to the control group. The average COD was also significantly lower. But positive TH reactions in the striata of the rTMS group mice were significantly greater than in the PD and srTMS groups, and the average COD was significantly higher. In the control group, the disposition of nerve fibers in the striatum was typically fasciculated, concentrated and ordered; in PD and srTMS groups, many fibers were lost, and the remaining nerve fibers were rare, ruptured and scattered; in the rTMS group fewer nerve fibers we

关 键 词：经颅磁刺激 黑质 纹状体 帕金森病 

分 类 号：R742.5[医药卫生—神经病学与精神病学]