脑梗死继发癫痫与GABABRl基因和PDYN启动子区基因多态性关系研究  

Association between polymorphisms of gamma-aminobutyric acid B receptor 1 gene and prodynorphin promoter region gene and epilepsy secondary to cerebral infarction

作　　者：王建平[1] 王利军[1] 蒋超[1] 王颖颖[1] 吴川杰[2] 

机构地区：[1]郑州大学第五附属医院神经内科,郑州450052 [2]郑州大学第一附属医院神经内科,郑州450000

出　　处：《中华神经医学杂志》2012年第12期1229-1232,共4页Chinese Journal of Neuromedicine

摘　　要：目的探讨γ-氨基丁酸B受体B1亚单位（GA BA BR1）基因（G1465A）多态性及前强啡肽原（PDYN）启动子区基因多态性与汉族人群脑梗死继发癫痫发生之间的关系。方法选择郑州大学第五、第一附属医院神经内科自2010年7月至2011年8月住院及门诊收治的67例脑梗死继发癫痫患者、同期收治的93例脑梗死无癫痫患者和104例体检正常者为研究对象．采集各组对象肘静脉血，柱层析法提取血细胞基因组DNA，聚合酶链式反应-限制性片段长度多态（PCR-RFLP）技术检测GA BA BRl基因（G1465A）多态性，聚合酶链式反应（PCR）技术检测PDYN启动子区基因多态性。结果3组研究对象酬鲋BRJ基因（Gl465A）都显示为G／G型，未发现A／G型和A／A型。脑梗死继发癫痫组中PDYN启动子区基因L／L型频率（76．1％）略高于脑梗死无癫痫组（73．2％）及正常对照组（73．1％），L型等位基因频率（85．8％）略高于脑梗死无癫痫组（85．5％）及正常对照组（84．6％），3组间基因型频率和等位基因频率比较差异均无统计学意义（P＞O．05）。结论GABABRJ基因（G1465A）及PDYN启动子区基因多态性与汉族人群脑梗死继发癫痫可能无明显关系。Objective To investigate the relationship between the polymorphisms ofgamma-aminobutyric acid B receptor 1 （GABABR1） gene （G1465A） and prodynorphin （PDYN） promoter region gene and epilepsy secondary to cerebral infarction in Han population. Methods Sixty-seven patients with epilepsy secondary to cerebral infarction, 93 patients diagnosed as having cerebral infarction without epilepsy and 104 healthy volunteers were selected in our hospitals from July 2010 to August 2011. EDTA-blood specimens were collected from test objects. DNA samples were extracted with column chromatography. The polymorphism of GABABR1 （G1465A） gene was detected by polymerase chain reaction - restriction fragment length polymorphism （PCR-RFLP） technique, and the polymorphism of PDYN gene promoter region was detected by PCR technique. Results Only genotype G/G was observed in the three groups without noting genotypes A/G and A/A in our study. The frequency of L/L genotype in patients with epilepsy secondary to cerebral infarction （76.1%） was slightly higher than that in patients with cerebral infarction without epilepsy （73.2%） and normal control group （73.1%） without significant difference （P〉0.05）. The frequency of L allele in patients with epilepsy secondary to cerebral infarction, patients with cerebral infarction without epilepsy and normal control group was 85.8%, 85.5% and 84.6%, respectively, without significant difference （P〉0.05）. Conclusion There may be no association between the polymorphisms of GA BA BR 1 （G 1465A） gene and PDYN promoter region gene and epilepsy secondary to cerebral infarction in Han population.

关 键 词：γ-氨基丁酸B受体B1亚单位 前强啡肽原 基因多态性 脑梗死 继发性 癫痫 

分 类 号：R742.1[医药卫生—神经病学与精神病学]