整合素α9β1对大鼠角膜缝线术后新生血管形成及血管内皮生长因子A表达的影响  

Effect of integrin α9β1 on corneal neovascularization and vascular endothelial growth factor A expression after corneal suture in rats

作　　者：吴静[1] 赵敏[1] 

机构地区：[1]重庆医科大学附属第一医院眼科,重庆400016

出　　处：《南方医科大学学报》2012年第12期1704-1707,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(81170822)~~

摘　　要：目的研究整合素α9β1对大鼠角膜缝线术后新生血管(CNV)形成及血管内皮生长因子A(VEGF-A)表达的影响并探讨其意义。方法 39只SD大鼠,随机分为正常组(3只),对照组、α9β1组、α9β1抑制组各12只。角膜行缝线术后,对照组予以左氧氟沙星点双眼;α9β1组予以左氧氟沙星+α9β1点双眼;α9β1抑制组予以左氧氟沙星+α9β1单抗Y9A2点双眼。术后每天在裂隙灯下观察CNV生长情况,并于3、5、7、14 d分别随机取3个实验组中各3只大鼠,在裂隙灯显微镜下行角膜照相,计算CNV面积;RT-PCR、Western blotting法检测不同时间点角膜中VEGF-A的表达。结果 3组大鼠缝线后均有CNV形成,在第7天CNV面积达最大,对照组CNV面积为3.21±0.19;和对照组比较,α9β1组CNV明显增多为4.57±0.31(P<0.05);而α9β1抑制组CNV明显降低,面积为2.03±0.26(P<0.05);第14天时CNV出现部分消退。RT-PCR和Western blotting检测结果示:正常角膜仅有少量VEGF-A表达,角膜缝线术后,VEGF-A表达明显增加。在术后第7天CNV生长达峰值;与对照组比较,α9β1组VEGF-A表达明显增高(P<0.05),而α9β1抑制组VEGF-A表达显著降低(P<0.05)。结论在角膜缝线术后,整合素α9β1可通过上调VEGF-A的表达促进CNV生成;给予整合素α9β1抑制剂可下调VEGF-A的表达和显著减少CNV生成。Objective To investigate the effect of integrin α9β1 on corneal angiogenesis and the expression of vascular endothelial growth factor A （VEGF-A） in rats after corneal suture. Methods Thirty-nine SD rats were randomly divided into normal control group, corneal suture model group, α9β1 group, and α9β1 suppression group, and in the latter 3 groups, the rats received topical administration of levofloxacin, levofloxacin ＋ α9β1, and levofloxacin＋ anti-α9β1 monoclonal antibody （Y9A2） twice daily after corneal suture, respectively. At 3, 5, 7, 14 days after the surgery, 3 rats were randomly selected from each group to observe corneal neovascularization （CNV） under slit lamp. RT-PCR and Western blotting were used to detect the expressions of VEGF-A. Results CNV was not observed in normal cornea. Neovascularization occurred in the corneal limbus in the 3 corneal suture groups, and the leak neovascularization count （CNVC）, which occurred on the 7th postoperative day, was significantly increased after α9β1 treatment compared with that in the model group （4.57±0.31 vs 3.21±0.19, P〈0.05） but markedly decreased after α9β1 suppression （2.03±0.26, P〈0.05）. VEGF-A was expressed at a low level in normal cornea, and increased significantly in the model group, reaching the peak level on 7th day （P〈0.05）; corneal VEGF-A expression was further enhanced by α9β1 treatment （P〈0.05） while significantly lowered by α9β1 suppression （P〈0.05）. Conclusion Integrin α9β1 can promote CNV by up-regulating VEGF-A expression and α9β1 suppression produces the opposite effect after corneal suture in

关 键 词：整合素α9β1 新生血管 角膜 缝线 血管内皮生长因子A 

分 类 号：R779.6[医药卫生—眼科]