红麻光钝感突变体光周期反应特性与RAPD扩增片段差异分析  

Photoperiod Reaction Traits of Light-insensitive Kenaf Mutant and Difference Analysis of Amplified Fragments with RAPD Marker

作　　者：徐建堂[1] 林荔辉[1] 祁建民[1] 张高阳[1] 方平平[1] 林培清[1] 池仁漫[1] 

机构地区：[1]福建农林大学作物遗传育种与综合利用教育部重点实验室,福州350002

出　　处：《中国麻业科学》2012年第6期260-264,共5页Plant Fiber Sciences in China

基　　金：国家麻类产业技术体系建设项目(CARS-19-E06);农业部东南黄红麻科学观测实验站建设项目(农科教发2011.9);福建省南方黄红麻种质资源创新平台项目(2010N2002);福建省自然科学基金(2011J05046)

摘　　要：为了研究红麻光钝感突变体基因的遗传规律及与红麻光钝感形成有关的基因序列特征。以红麻品种福红952经航天诱变获得的光钝感突变体与光敏感红麻细胞质保持系L23B品种杂交,杂交后代即F2群体在海南130d短日照条件下诱导了花蕾的分化发育,统计其分离情况,同时利用经筛选的多态性较好的RAPD引物扩增光钝感红麻与对照品种基因组DNA。结果表明:光钝感与光敏感性状存在一对基因的遗传差异,红麻光周期钝感性状为隐性遗传。从24个多态性较好的RAPD引物中筛选到3个引物可以把红麻光钝感突变体与对照品种区分开来,其中有1条(1178bp)是编码蛋白的基因片段,说明RAPD不仅扩增基因组上的非编码蛋白序列,同时可扩增编码蛋白的基因片段,反映不同红麻光钝感材料遗传上的差异。该研究结果可为进一步开展红麻光钝感的基因克隆及分子机理研究提供重要的遗传材料。This paper aimed at studying the genetic features of kenaf light - insensitive mutant. A light - insensitive mutant which was from Fuhong 952 mutated in aerospace, and a light sensitive cyto- plasmic maintainer L23 B were crossed as parents, the F2 population was induced to differentiate buds for 130 d under short -day condition in Hainan province, and the genetic separation of the F2 was analyzed. At the same time, the screened polymorphism RAPD primers were used to amplify genornic DNAs of the light insensitive mutant and the CK. The results showed that there existed three phenotypes in F2 genera- tion, and the separation ratio was 3 ： 1 of the light sensitive to the light insensitive ones.

关 键 词：红麻 光钝感突变体 光周期反应 RAPD 特异片段 

分 类 号：S563.5[农业科学—作物学]