老鹳草中药材红外光谱的分析与鉴定  

Analysis and Identification of Geranium by Two-Dimensional Correlation Infrared Spectroscopy

作　　者：孙仁爽[1] 金哲雄[1] 张哲鹏[1] 许长华[2] 孙素琴[2] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心,黑龙江哈尔滨150076 [2]清华大学化学系,北京100084

出　　处：《光谱学与光谱分析》2013年第1期81-84,共4页Spectroscopy and Spectral Analysis

摘　　要：采用红外光谱、二阶导数光谱和二维相关红外光谱对来自黑龙江、吉林、辽宁三省的老鹳草中药材进行了分析。不同产地的老鹳草的红外光谱都具有1 730和1 337cm-1等表征鞣质类成分的特征吸收峰和1 618和1 318cm-1波数表征草酸钙的特征峰。其中还发现在1 370cm-1的C—H弯曲振动区和1 230cm-1的C—O伸缩振动区三产地老鹳草具有一定的差异性。在高分辨的二阶导数谱中,1 509,1 204,764和763cm-1附近的表征鞣质类成分的吸收峰更明显,三产地老鹳草在CO羰基伸缩振动区表征了脂肪类化合物的不同;尤其是在二维相关光谱上,各自的自动峰具有显著的差异性,黑龙江的老鹳草出现了7个自动峰,其中1 621cm-1处自动峰最强;而在吉林和辽宁产地的老鹳草中同样出现了4个自动峰,前者1 580cm-1处的自动峰最强,后者在1 649cm-1处自动峰最强。该方法直观、简单、方便、快速,可以作为鉴别和鉴定同种不同产地老鹤草的一种新方法。Tri-step infrared spectroscopy（Fourier transform infrared spectroscopy（FTIR） combined with second derivative spectra and two-dimensional correlation infrared spectroscopy（2D-COS）） was employed to identify and analyze the main components of Heilongjiang（HLJG）,Jilin（JLG）,Liaoning（LNG） genuine Herba Geranium.The emergence of several characteristic absorption peaks of tannins including 1 730 and 1 337 cm-1 and peaks around 1 618 and 1 318 cm-1 belonging to calcium oxalate suggested that Herba Geranii contained tannins and calcium oxalate.Differences near 1 370 and 1 230 cm-1 were found among the three Herba Geranii.In light of second derivative spectra,four more peaks of tannin components around 1 509,1 204,764 and 763 cm-1 and evident differences around CO stretching bands（1 750~1 600 cm-1） were observed.By 2D-COS spectra with further improved resolution,the three genuine Geraniums were visually distinguished due to their significant differences in auto-peak profile.HLJG has 7 auto peaks with a strongest peak around 1 621 cm-1,while JLG and LNG both have only 4 auto peaks with a strongest peak around 1 580 and 1 659 cm-1,respectively.It was demonstrated that the Tri-step infrared spectroscopy was successfully applied to fast analyze and identify genuine Geraniums from different geographical regions and subsequently would be applicable to the study of Chinese medicinal resources and quality standards.

关 键 词：老鹳草 红外光谱 二阶导数光谱 二维相关红外光谱 

分 类 号：O657.3[理学—分析化学]