脑神经生长素治疗兔急性高眼压视神经损伤  

Experimental study of the effects of CNG on the optic nerve injured by acute ocular hypertension

作　　者：王传富[1] 潘晓晶 金梅玲[1] 张世杰[1] 姜涛[1] 张海平[2] 

机构地区：[1]青岛医学院附属医院眼科,266003 [2]青岛市立医院眼科

出　　处：《中华眼底病杂志》2000年第2期88-90,共3页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的　观察脑神经生长素 (cranial nerve growthine,CNG)对兔眼急性高眼压视神经损伤的治疗作用。　方法　健康成年新西兰大耳白兔 (体重 2 .5～ 3.0 kg) 30只 ,随机分为 5组 ,每组 6只 ,一只眼前房内灌注生理盐水使眼压升高到 5 0 mm Hg(1mm Hg=0 .133k Pa) ,并维持 6 h,另一只眼为自身对照眼。每组 3只兔制成高眼压模型眼后肌肉注射 CNG0 .2 ml/ d作为治疗组 ,其余 3只兔为实验组。 5组兔分别于制造模型眼后 1、3、7、15、30 d处死。用辣根过氧化物酶 (horserdish peroxidase,HRP)组织化学染色法和透射电镜技术观察 CNG对急性高眼压视神经顺行轴浆运输和超微结构的影响 ,定量分析 CNG的药物疗效。　结果　 7d后 ,治疗组 HRP反应物积分光密度 (39.79± 2 .2 9)较实验组 (2 5 .17± 1.0 3)明显增多 ,差异有显著性意义 (P<0 .0 1) ;治疗组视神经轴突变性较实验组轻 ,15 d后 ,部分轴突结构恢复正常 ,线粒体增多 ,30d后 ,轴突结构基本正常。　结论　CNG可改善急性高压眼视神经轴浆运输状况 ,促进视神经结构的恢复。Objective [WT5”BZ]To observe in vitro the protective effect of cranial nerve growthine (CNG) on the optic nerve injured by acute ocular hypertension. [WT5”HZ]Methods Thirty white rabbits were divided into five groups,and 6 in each.The acute ocular hypertension (50 mm Hg) models were established by forcing perfusion of normal saline solution into the anterior chamber sustained for 6 h in one eye,and the contral ateral eye of each rabbit was regard as control.Three rabbits in each group were then treated by CNG 0.2 ml intramuscularly every day.The optic nerve and retina was surgically removed at five different time points (lst,3rd,7th,15th and 30th day) after operation.With the HRP orthograde tracing technique and transmitted electron microscope,the effect of CNG on the optic nerve was observed by the changes of axonal transport and ultrastructure of optic nerve. [WT5”HZ]Results [WT5”BZ]Compare with experimental control groups (25.17± 1.03), HRP reactive products of treated groups (39.79±2.29) markedly increased after seven days (P< 0.01). The degeneration of axons in treated groups was relatively lighter after fifteen days and some axons recovered after thirty days. [WT5”HZ]Conclusion [WT5”BZ]CNG might improve the axonal transport and the recovery of axons after the optic nerve injured by acute ocular hypertension. [WT5”HZ]

关 键 词：高眼压 视神经损伤 脑神经生长素 兔 

分 类 号：R774.6[医药卫生—眼科]