冠状动脉内放射对PDGF-B和iNOS基因表达以及NO的影响  

EFFECTS OF INTRACORONARY RADIATION ON THE EXPRESSION OF PDGF-B mRNA, iNOS mRNA,AND NO AFTER CORONARY ARTERY BALLOON INJURY IN SWINE

作　　者：何昆仑[1] 盖鲁粤[1] 黄大显[1] 高焱章[1] 王所亭[1] 

机构地区：[1]中国人民解放军总医院,北京100853

出　　处：《解放军医学杂志》2000年第3期162-164,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助项目!(编号39900059)

摘　　要：为探讨冠状动脉内放射抑制再狭窄的机制，对２３头小型猪冠状动脉行球囊过度扩张术，其中１２头在术后即刻通过^（１９２）Ｉｒ血管内放射治疗系统局部给予２０Ｇｙ的放射剂量，其余１１头作为对照组。用反转录－聚合酶链反应（ＲＴ－ＰＣＲ）方法定量检测血管组织血小板源生长因子（ＰＤＧＦ－Ｂ）和诱导型一氧化氮合成酶（ｉＮＯＳ）基因表达，并测定血管组织一氧化氮（ＮＯ）的含量。结果：冠状动脉内放射明显抑制了术后３天和术后３０天 ＰＤＧＦ－ＢｍＲＮＡ表达（３８．３％，Ｐ＜０．０１；３９．４％，Ｐ＜０．０５）；明显增加了球囊扩张术后３天及术后３０天血管组织ｉＮＯＳｍＲＮＡ的表达（３２．５％，Ｐ＜０．０１；３４．２％，Ｐ＜０．０５）和ＮＯ的含量（１０２．０％，Ｐ＜０．０１；９０．８％，Ｐ＜０．０１）。To investigate the mechanisms of intracoronary radiation in inhibiting restenosis after coronary artery balloon injury in swine. Twenty-three Chinese swines underwent coronary balloon over-stretch. The catheter- based radiation system was used to deliver 20Gy to the dilated segments in 12 swines, and 11 swines were used as control group. The animals were sacrificed three and thirty days following the initial procedure. The injured segments were dissected and processed to examine the gene expression of iNOS and PDGF-B by reverse transcription- polymerase chain reaction (RT-PCR) and to measure the production of NO by biochemistry. RESULTS:Intracor- onary radiation significantly reduced PDGF-B mRNA (38. 3 %, P<0. 01; 39. 4%, P<0. 05 ) and increased iNOS mRNA(32. 5 %, P<0. 01 ; 34. 2%,P<0. 05) three days and thirty days after the initial injury. Furthermore,in- tracoronary radiation also increased the production of NO three and thirty days after the injury (102. 0%, P<0. 01; 90. 8%,P<0. 01). CONCLUSIONS: PDGF-B and NO system may be involved in the mechanisms by which intracoronary radiation inhibits restenosis after balloon injury.

关 键 词：冠状动脉 放射疗法 PDGF-B 一氧化氮 INOS 

分 类 号：R654.202[医药卫生—外科学] R816.2[医药卫生—临床医学]