实体瘤患者外周血造血干细胞的动员与保存  

Mobilization and Preservation of Peripheral Stem Cells in Solid Tumors

作　　者：刘宗印[1] 孙玉萍[1] 肖东杰[1] 张红[1] 李均[1] 赵建强[1] 孙善会[1] 

机构地区：[1]济南市中心医院,济南市250013

出　　处：《中国肿瘤临床》2000年第6期405-407,共3页Chinese Journal of Clinical Oncology

基　　金：山东省济南市科委科研基金

摘　　要：目的 :探讨一种适于晚期恶性肿瘤治疗的自体外周血造血干细胞移植方法。方法 :单用rG -CSF(75μg,q12h×2d)作动员剂 ,用血细胞分离机一次性收集单个核细胞(MNC),4℃贮存。观察WBC、MNC计数、细胞活力及细胞分类 ;用流式细胞仪进行细胞表型检测。结果 :小剂量rG -CSF动员后 ,外周血WBC达(18.71±6.54)×109/L。一次可收集MNC(1.87±0.66)×108/kg。CD34、CD14、CD3 及CD22 细胞分别为4.39 %、3.85 %、4.63 %及3.76 %。CD34 细胞平均约为8.2×106/kg。4℃贮存48～56小时MNC存活率为94.3 %。结论 :对实体瘤尚无骨髓转移的患者 ,用此方法可获得足以重建造血功能的干细胞。Objective: To probe the way of autologous peripheral blood stem cell transplantation (APBSCT) for treatment of advanced malignant tumor. Methods: The peripheral blood stem cells were mobilized by rG-CSF (75ug q12h x 2d) and isolated by CS-3000 Plus Blood Cell Seperator and stored at 4℃ for 48～56 hrs. The number of WBC and MNC and cell vibility were counted and classified by BVPM multi-microscope and CD34, CD14, CD3 and CD22 were determined by flow microfluorometer. Results:After mobilization by G-CSF, the number of WBC increased to 18.71±6.54×109/L (averagely 3.23 times of that before mobilized). MNC was 1.87±0.66×109 Kg and CD34 cell number was 8.2×106/Kg. The positive rates of CD34, CD14, CD3 and CD22 were 4.39%, 3.85%, 4.63% and 3.76% respectively. The survival rate of MNC at 4℃ was 94.3%. Conclusion: The results showed that mobilized APBSC stored at 4℃ is helpful for the patients with solid malignant tumor to get reconstructed hemopoietic function under high dose chemotherapy.

关 键 词：实体瘤 外周血造血干细胞移植 动员剂 冷冻保存 

分 类 号：R73-3[医药卫生—肿瘤]