成骨性肿瘤中p27基因mRNA与蛋白的表达及其意义  

Significance of p27mRNA and Protein Expression in Osteoplastic Tumors

作　　者：文彬[1] 丘钜世[2] 李扬[2] 

机构地区：[1]四川省川北医学院病理教研室 [2]中山医科大学病理教研室,广州市510080

出　　处：《中国肿瘤临床》2000年第6期414-417,共4页Chinese Journal of Clinical Oncology

基　　金：美国中华医学基金会(CMB)资助!(编号 :93~594)

摘　　要：目的 :探讨 p27mRNA与蛋白在成骨性肿瘤中的表达状况 ,了解其与肿瘤组织学类型、分化程度及生物学行为的关系。方法 :采用免疫组化和非放射性核酸原位杂交技术检测成骨性肿瘤中 p27蛋白和mRNA的表达状况。结果 :骨瘤、骨母细胞瘤中 p27蛋白高表达 ,主要定位于胞核 (85.7 %、75 % ) ,骨肉瘤中低表达 ,胞核表达极低(4.7 % ) ,两者间差异显著 (P<0.01)。p27蛋白在骨肉瘤中的表达与肿瘤的浸润、复发和转移有关。原位杂交显示良、恶性成骨性肿瘤的 p27mRNA表达阳性率及表达强度无显著性差异 (P>0.05)。且与骨肉瘤的组织学类型、分级及生物学行为无关。结论 :p27蛋白表达在成骨性肿瘤中的改变具有特异性 ,可作为骨肉瘤的诊断和评估预后的指标。骨肉瘤中p27蛋白表达低下可能是由于转录后特异性的蛋白酶介导的降解所致。Objective: To evaluate the significance of p27mRNA and protein expression in various kinds of osteoplastic tumors. Methods: Immunohistochemistry and nonradioactive in situ hybridization methods were used to detect the expression of p27mRNA and protein in 58 cases of osteoplastic tumors. Results: The positive rate of p27 protein was 100% in osteoma, 87.5% in osteoblastoma, 62.8% in osteosarcoma. In addition, the nuclear staining of p27 was noted 85.7% (6/7) in osteoma, 75% (6/8) in osteoblastoma, but only 4.7% (2/43) in osteosarcoma. The results showed a statistical significance between benign tumors and osteosarcoma (P< 0.01). The expression of p27 protein in osteosarcoma was significantly correlated with the invasivenes, relapse and metastasis of tumor. In situ hybridization, it revealed that there was no difference of expression levels of p27 mRNA between benign tumors and osteosarcoma and there was no statistical significance between p27 mRNA expression and its histological types, differentiation, degree and biological behavior of osteosarcoma. Conclusion: p27 protein has practical value in diagnosis of osteosarcoma, and it mayserves as a new biological indicator to predict the prognosis of the patient with osteosarcoma. Loss of p27 protein of osteosarcoma may be resulted from a posttranscriptional specific proteosorne-mediated degradation.

关 键 词：成骨性肿瘤 MRNA P27基因 P27蛋白 基因表达 

分 类 号：R738.1[医药卫生—肿瘤]