纳秒脉冲电场治疗裸鼠皮下人恶性黑色素瘤模型的长期效应  

Long-term Effects of Nanosecond Pulsed Electric Field on Nude Mouse Model with Human Melanoma

作　　者：姚陈果[1] 郭飞[1] 王建[1] 孙才新[1] 赵雪[2] 唐均英[2] 

机构地区：[1]重庆大学输配电装备及系统安全与新技术国家重点实验室,重庆400030 [2]重庆医科大学附属第一医院,重庆400016

出　　处：《高电压技术》2012年第12期3357-3362,共6页High Voltage Engineering

基　　金：国家自然科学基金(50637020;50877083);教育部博士点基金(20100191110019)~~

摘　　要：为研究ns脉冲电场(nsPEF)对在体肿瘤的长期杀伤效应,以接种人恶性黑色素瘤A375细胞的BALB/c裸鼠为研究对象,采用电压幅值为4kV、脉冲宽度为200ns、重复频率为1Hz的ns脉冲电场进行治疗。肉眼观察结果表明,瘤体随时间推移而逐渐消褪直至消失,且皮肤恢复完好,原位瘤在平均2周时间内完全消失;瘤体生长抑制情况检测结果表明,与对照组相比,治疗组裸鼠肿瘤体积明显缩小(检验水准P<0.05),随着生存时间延长,对照组和治疗组肿瘤生长速度差别增大;荷瘤裸鼠的存活率-时间关系研究结果表明,ns脉冲电场治疗后26d时间内,对照组裸鼠全部死亡,而治疗组裸鼠存活率高达100%。实验结果表明ns脉冲电场能有效抑制肿瘤组织的生长,并可大大提高荷瘤BALB/c裸鼠的长期存活率。To investigate the long-term effect of nanosecond pulsed electric field （nsPEF） on in vivo tumor tissue, by selecting BALB/c nude mice inoculated with human melanoma cells as target, nsPEF with amplitude of 4 kV, pulse width of 200 ns, repetition frequency of 1 Hz was performed on target mice, and the treatment effect was studied. According to perusal results, after the nsPEF treatment, the volume of the tumor tissue decreased with the time till the tumor disappeared in an average of two weeks with no fatal damage to the skin. Compared with the control group, the tumor volume in the treated group significantly decreased { an inspection level less than 0.05）, and the differences between the tumor volumes of treated group and control group increased along with the surviving time; survival rate-time relationship indicated that all mice in the control group died within 26 days, whereas, no mice death was observed in the nsPEF treated group. Therefore it is concluded that nsPEF has a significant growth inhibition effect on tumor tissues, and the survival of BALB/c nude mouse can be greatly enhanced.

关 键 词：ns脉冲电场(nsPEF) 杀伤效应 人恶性黑色素瘤细胞 BALB c裸鼠 生长抑制 存活率 

分 类 号：R739.5[医药卫生—肿瘤]