成人急性白血病中p73基因的表达及意义  

Expression and significance of p73 in adults with acute leukemia

作　　者：潘少君[1] 王俊和[1] 张凤[1] 

机构地区：[1]蚌埠医学院第二附属医院,233000

出　　处：《实用医学杂志》2012年第24期4076-4078,共3页The Journal of Practical Medicine

基　　金：安徽省卫生厅医学科研基金资助项目(编号:2010C082)

摘　　要：目的:检测并分析p73在急性白血病(AL)患者骨髓单个核细胞(BMMNC)中的表达情况,初步探讨p73与p53的相关性以及p73和细胞抗原表达的相关性,从而分析p73在AL中的临床意义。方法:应用逆转录聚合酶链反应(RT-PCR)检测AL组和对照组BMMNCp73与p53的表达情况。采用流式细胞术对ALBMMNC细胞表面及浆内分化抗原检测,分析p73与细胞抗原的相关性。结果:ALL初诊组p73阳性表达率低于ALL-CR组和对照组(P<0.05),AML初诊组p73阳性表达率明显高于AML-CR组和对照组(P<0.05)。30例AML初诊组中p73与p53、CD2呈正相关,与MPO(髓过氧化物酶)呈负相关(r=0.481,P<0.01;r=0.447,P<0.05;r=-0.492,P<0.01)。20例ALL初诊组中,p73与p53无相关性(P>0.05),p73与细胞抗原表达亦无相关性(P>0.05)。结论:p73在AL发生发展和预后中可能发挥重要作用。Objective To determine the expression of p73 in bone marrow mononuclear cell （BMMNC） of patients with acute leukemia （AL）, to study the correlations between p73 and p53, and between p73 and antigen presentation intensity, and to explore the corresponding clinical significance of p73. Methods The expressions of p73 and p53 in BMMNC of patients with AL and of controls were determined by RT-PCR. The immunophenotypes of BMMNC of patients with AL were assayed by flow cytometry. Results The positive expression rate of~ p73 in initially diagnosed ALL group was lower than those in ALL-CR group and control group （P 〈 0.05）, that in initially diagnosed AML group was higher than those in AML-CR group and control group （P 〈 0.05 ）. The expression of p73 was positively correlated with p53 （r = 0.481, P 〈 0.01） and CD2 （r = 0.447, P 〈 0.05）, and was negatively correlated with MPO （r = -0.492, P 〈 0.01 ） in 30 cases of initially diagnosed AML group; while that was not correlated with p53 and antigens in 20 patients of initially diagnosed ALL （P 〉 0.05）. Conclusion p73 may play an important role in the occurrence and prognosis of AL.

关 键 词：急性白血病 P73 RT—PCR 流式细胞术 预后 

分 类 号：R733.71[医药卫生—肿瘤]