^(103)钯放射性支架诱导胆管癌细胞凋亡及对抑癌基因的影响和意义  

Significance of ^(103)pd Radioactive Stent Induced Cholangiocarcinoma Cells Apoptosis and Effects on Tumor Suppressor Genes

作　　者：何贵金[1] 纪大伟[1] 顾玺[1] 王柳[1] 赵晓丹[1] 潘春来[1] 李安[1] 许书河[2] 高沁怡[3] 姜维国[4] 郭启勇[5] 

机构地区：[1]中国医科大学附属盛京医院普外科,沈阳110004 [2]中国原子能研究院同位素研究室,北京100050 [3]中国医科大学附属第一医院同位素室,沈阳110001 [4]中国医科大学附属盛京医院病理科,沈阳110004 [5]中国医科大学附属盛京医院放射科,沈阳110004

出　　处：《中国医科大学学报》2012年第12期1099-1101,共3页Journal of China Medical University

基　　金：辽宁省自然科学基金资助项目(20042063);辽宁省教育厅高校科研计划(05L445);辽宁省科技计划项目(2006225001-6);沈阳市科技计划项目(F12-193-9-01)

摘　　要：目的探讨103钯(103pd)放射性支架对p53抑癌基因在辐射诱导胆管癌细胞凋亡中的影响和意义。方法 12只裸鼠随机分2组,每组6只,在裸鼠胆管癌内分别植入103pd放射性支架和普通胆管支架,术后30 d取出胆管癌标本进行HE染色和p53基因原位杂交,DAB染色p53基因表达阳性者细胞质呈棕黄色,胆管癌细胞凋亡染色细胞核中有棕黄色浓染的为阳性细胞。结果103pd放射性支架组胆管癌细胞p53基因阳性表达率为89.63%,而普通支架组为21.76%,2组比较差异有统计学意义(P<0.01);103pd放射性支架组胆管癌凋亡细胞数明显大于普通支架组,2组比较差异有统计学意义(P<0.05)。结论103pd放射性支架可以激活p53基因,使其功能提高,诱导胆管癌细胞凋亡。Objective To investigate the role of tumor suppressor genes （p53 gene） in radioactive induced cholangioarcinoma cells apoptosis. Methods A total of 12 nude mice were randomly divided into 103pd radioactive stent group （n =6） and control group （n =6）. Cross-sections were stained by HE for cholangiocarcinoroa. The expression of p53 in cholangiocarcinoma tissue was detected using in situ hy- bridization,cells with the brow-yellow nucleus ＇after DAB coloration indicates positive staining. Positive rate was analyzed under micrescope and apoptosis cell in paraffln-bedding cholangiocarcinoma tissue section was determined using immunohistological staining method. Results Expression of p53 was higher in the 103pdradioactive stem group than that in the control group （P 〈 0.01 ）. The cholangiocarcinoma cell apoptosis rate was significantly increased in the 103pd radioactive stent group than the control group （P 〈 0.01 ）. Conclusion These results suggest that p53 gene was increased by 103pd and induced cholangicareinoma cell apoptosis,the results ~so show that 103pd radioactive stent can inhibit cholangiocarcinoma cell proliferation.

关 键 词：P53基因 细胞凋亡 Γ射线 

分 类 号：R735.8[医药卫生—肿瘤]