不同根管消毒药物对粪肠球菌杀菌效果的体外研究  

Evaluation of in vitro Antibacterial Effect of Root Canal Dressing on Enterococcus Faecalis

作　　者：王雪梅[1] 王戈[2] 仇丽鸿[1] 于静涛[1] 

机构地区：[1]中国医科大学口腔医学院牙体牙髓科,中国医科大学口腔医学院中心实验室,辽宁省口腔医学研究所牙体牙髓病学研究室,沈阳110002 [2]鞍山市中心医院口腔科,辽宁鞍山114001

出　　处：《中国医科大学学报》2012年第12期1113-1115,共3页Journal of China Medical University

基　　金：辽宁省科学技术计划项目(2010225001);中国医科大学口腔医学院青年科研启动基金(K101593-10-23)

摘　　要：目的比较不同根管消毒药物对于粪肠球菌感染根管的抗菌效果。方法选用新鲜拔除的60颗前牙,ProTaper预备至F3,将粪肠球菌标准菌株(ATCC29212)接种在无菌根管内培养建立感染模型,随机分为4组,分别用生理盐水(A组)、Vitapex(B组)、2%洗必泰(C组)、2%洗必泰凝胶(D组)进行根管封药,7 d后接种在BHI琼脂平皿上计数菌落。结果封药前4组根管内细菌量的差异无统计学意义(P>0.05)。A组封药前后根管内细菌量无统计学差异(P>0.05),B、C、D组封药后根管内细菌量明显减少,与封药前比较差异有统计学意义(P<0.05)。C、D组与B组比较,封药后根管内细菌量明显减少,封药前后细菌量的差值明显升高(P<0.05),但C组与D组比较,封药后根管内细菌量及封药前后细菌量的差值无统计学差异(P>0.05)。结论2%洗必泰凝胶是再治疗根管最佳的消毒药物。Objective To evaluate the antibacterial efficacy of root canal dressing using an in vitro model with Enterococcusfaecalis （E. faecah＇s）. Methods After biomechanical instrumentation, the root canals of sixty extracted teeth were infected by E. faec^is in vitro. The sixty infected teeth were randomly divided into 4 groups. All root canals were dressed for 7 days with different root canal ＆dressing methods in- cluding sodium chloride, Vitapex, 2% chlorhexidine, and 2% chlorhexidine gelatin. Then, the samples were inoculated in BHI agar and the colony of bacteria was counted （CFU/mL）. Results The number of E. facealis in group of sodium chloride was significantly higher than other groups （P 〈 0.05）, while the number of E. faecalis in group Vitapex was significantly higher than group of 2% chlorhexidineand and 2% chlorhexidine gelatin （P 〈 0.05）. There was no significant difference between group 2% chlorhexidine and group 2% chlnrbexidine gelatin. Conclusion The results suggested 2% chlorhexidine gelatin as the best drug for root canal dressing.

关 键 词：洗必泰凝胶 根管消毒 粪肠球菌 

分 类 号：R781.05[医药卫生—口腔医学]