乳腺癌细胞核差异蛋白筛选  

Screening for differential Proteins of Cell Nuclear among Breast Cancer

作　　者：韵雪雪[1] 杨永长[1] 姜伟[1] 肖代雯[1] 闫慧[2] 刘华[1] 黄文芳[1] 

机构地区：[1]四川省医学科学院·四川省人民医院,四川成都610072 [2]重庆医科大学医学检验系,临床检验诊断学教育部重点实验室

出　　处：《现代预防医学》2012年第24期6442-6445,共4页Modern Preventive Medicine

基　　金：国家"863"计划(2006AA02090408)资助项目

摘　　要：目的比较体外培养的正常乳腺细胞和乳腺癌细胞核蛋白指纹图谱,筛选差异蛋白,为乳腺癌亚细胞蛋白标志物的研究奠定基础。方法提取正常乳腺细胞株HBL-100和乳腺癌细胞株MCF-7、MDA-MB-231的核蛋白,表面增强激光解吸电离飞行时间质谱(SELDI-TOF-MS)检测蛋白表达,重复试验30次,Biomaker Wizard Software分析软件统计结果并筛选差异蛋白。结果分子量(Mr)2～100kD范围内MCF-7和MDA-MB-231与HBL-100相比差异蛋白点数目分别为78个和69个(P﹤0.05),其中5.8kD、8.3kD和18.2kD的蛋白在两株癌细胞中表达均降低,9.9kD和15.7kD的蛋白在两株癌细胞中表达均增高。结论 SELDI-TOF-MS能够快速灵敏地检测分析乳腺癌细胞核差异表达蛋白,为乳腺癌亚细胞蛋白标志物的研究提供了新思路。OBJECTIVE To screen differential nuclear proteins by comparing protein fingerprinting between normal breast ep- ithelial and breast cancer cell lines cultured in vitro, which may lay a foundation for sub-cellular protein biomarker of breast cancer. METHODS Nuclear proteins of one normal breast epithelium cell line HBL-100 and two breast cancer cell lines MCF- 7 as well as MDA-MB-231 were extracted, and protein finger printings were measured by surface enhanced laser desorption ＆ ionization time-of-flight mass spectrometry （SELDI-TOF-MS） for 50 times. Differential proteins were analyzed and screened by Biomaker Wizard Software. RESULTS Seventy eight and sixty nine differential nuclear protein peaks showed significantly differ- ence between MCF-7 and MDA-MB-231 when compared with HBL-100 （P〈 0.05）, protein peaks at 5.8kD, 8.3kD and 18.2 kD were down-regulated in both breast cancer cell lines, while protein peaks at 9.9kD and 15.7kD were up-regulated. CON- CLUSION SELDI-TOF-MS can detect nuclear proteins of breast cancer cell lines accurately and sensitively, which applies a new method for sub-cellular protein biomarkers of breast cancer.

关 键 词：乳腺癌 细胞核蛋白 SELDI-TOF-MS 蛋白指纹图谱 差异蛋白 

分 类 号：R737.9[医药卫生—肿瘤]