DNA含量及S期细胞比值和胃癌生物学特性的关系  

Relation of DNA Content and S phase Fraction to the Biological Characteristic of Gastric Carcinoma

作　　者：周烨[1] 周决[1] 施达仁[1] 朱慰祺[1] 

机构地区：[1]上海医科大学附属肿瘤医院,200032

出　　处：《实用癌症杂志》2000年第2期121-123,共3页The Practical Journal of Cancer

摘　　要：目的　研究DNA含量及SPF值与胃癌临床病理指标及PCNA表达的关系。方法　应用流式细胞术测定 72例胃癌新鲜组织及 3 1例良性胃粘膜病变新鲜组织的DNA含量及SPF值 ,并采用免疫组织化学方法检测 66例胃癌石蜡切片的PCNALI ,并结合临床资料分析其临床意义。结果　胃癌组的DI( 1.2 1)及异倍体检出率 ( 4 4.44%)明显高于良性胃粘膜病变组 (分别为1.0 5和 9.68%)。胃癌患者中异倍体组的SPF值 ( 2 2 .74%)及PCNALI( 66.12 %)明显高于二倍体组的SPF值 ( 15 .5 8%)及PC NALI( 61.0 3 %) ,高SPF值组的异倍体检出率 ( 67.86%)及PCNALI( 66.89%)明显高于低SPF值组的异倍体检出率 ( 2 1.0 5 %)及PCNALI( 60 .3 4 %)。DNA倍体与患者年龄及肿瘤部位有关 ,SPF值仅与肿瘤部位有关 ,两者均与其他临床病理指标无相关性。结论　胃癌DNA倍体及SPF值均与肿瘤的增殖活性有关。Objective To investigate the relation of DNA content and SPF to the clinicopathological characteristic and the expression of PCNA in gastric carcinoma.Methods DNA content and S phase fraction were determined by flow cytometry in 72 cases of gastric cancer tissue and 31 cases of gastric benign lesion and PCNA expression was detected by immunohistochemistry in paraffin embedded sections in 66 tumor specimens.Results The incidence of DI and aneuploid in gastric tumor(1.21 and 44.44%,respectively) were significantly higher than those in gastric benign lesion(1.05 and 9.68%,respectively).Aneuploid tumors showed higher SPF(22.74%) and PCNA LI(66.12%) incidence than diploid tumors(15.58% and 61.03%,respectively).The incidence of aneuploid and PCNA LI in patients with high SPF were 67.86% and 66.89% respectively,while in patients with low SPF they were 21.05% and 60.34% respectively.DNA ploidy was related to age of patients and tumor location,while SPF showed relative only to tumor location.Conclusion DNA ploidy and SPF are closely related to the proliferative activity in gastric carcinoma.

关 键 词：胃癌 DNA含量 S期细胞比 增殖细胞核抗原 生物学 

分 类 号：R735.2[医药卫生—肿瘤] R730.23[医药卫生—临床医学]