紫外线照射对钛种植体不同管径TiO2纳米管涂层抗菌性能的影响  

Effects on the antibacterial activity of TiO2 nanotubes with different diameters from ultraviolet ray-irradiation

作　　者：杨惠铃[1] 梅盛林[1] 黄萍[2] 张玉梅[1] 

机构地区：[1]第四军医大学口腔医学院修复科,西安710032 [2]第四军医大学口腔医学院检验科

出　　处：《中华口腔医学杂志》2012年第12期748-752,共5页Chinese Journal of Stomatology

基　　金：国家自然科学基金（81070862、31170915）

摘　　要：目的研究紫外线照射对钛种植体不同管径YiO2纳米管涂层表面特性及抗菌性能的影响，为纳米管涂层的临床应用奠定基础。方法在5、10和20V电压下阳极氧化制备TiO2纳米管试样，以抛光纯钛为对照组，紫外线照射24h后，场发射扫描电镜观察试样表面形貌；测量去离子水、二碘甲烷和甲酰胺在试样表面的接触角；选用金黄色葡萄球菌采用薄膜密着法观察试样抗菌性能；场发射扫描电镜观察细菌形态；激光共聚焦显微镜观察细菌在试样表面的黏附及胞膜损伤情况并进行荧光强度分析。结果5、10和20V电压可制备管径约30、50和100nm的纳米管涂层。紫外线照射后不同管径TiO2纳米管涂层表面形貌无变化，随着管径增大，各涂层组接触角变小，细菌总荧光强度和死菌／活菌比值均增大，20V组总荧光强度最大（26．550±2．940）；死菌／活菌比值最高（0．728±0．091），显著高于其他组（P〈0．05）。结论紫外线照射虽然促进金黄色葡萄球菌的黏附，但可增强不同管径TiO2纳米管涂层的抗菌作用。紫外线照射后20V组涂层的抗菌性能最佳。Objective To study the effects of uhraviolet ray （UV）-irradiation on the surface characteristic and antibacterial activity of TiO2 nanotubes with different diameters. Methods TiO2 nanotubes with different diameters were fabricated on polished pure titanium（PT） samples by anodization at 5, 10 and 20 V with PT as control. The samples were exposed to UV-irradiation for 24 h, then the characteristic and antibacterial activity were analyzed and evaluated. The surface topograph was observed by field emission scanning electron microscope（FE-SEM）. Contract angle measurements were carried out with three liquids. Staphylococcus aureus （Sa） were used to evaluate the antibacterial activity of samples with the film contact method. The bacterial morphology was observed by FE-SEM. The bacterial adhesion and cell membrane injury were evaluated by fluorescent staining analysis under laser scanning confocal microscope （LSCM）. Results After the TiO2 nanotubes with different diameters were exposed to UV-irradiation, no change was observed in its surface topograph. With the increase of the diameters of nanotubes, each contract angle of nanotubes decreased, and bacterial Fit and dead/live ratio were also increased. We found 20 V Fit was the biggest（26. 550 ＋ 2. 940） and ranks the highest ratio of death/live （0. 728 ＋ 0. 091 ） among the others （P 〈 O. 05 ）. Conclusions The UV-irradiation can decrease the contract angle of TiO2 nanotubes and promote the Sa adhesion on nanotubes. Meanwhile, the antibacterial activity of TiO2 nanotubes with different diameters was remarkably enhanced by UV-irradiation. Nanotubes anodized at 20 V showed the best antibacterial activity.

关 键 词：紫外线 纳米管 钛 葡萄球菌 金黄色 

分 类 号：R783.6[医药卫生—口腔医学]