凉血活血药物对氧诱导小鼠视网膜新生血管实验中HIF-1和NF-κB的影响  

Effects of Liangxuehuoxue Drugs on Hyperoxia Induced Retinal Neovascular HIF-1 and NF-кB Expression in Mice

作　　者：马珊[1] 王明芳[2] 

机构地区：[1]云南中医学院第一附属医院眼科,云南昆明650021 [2]成都中医药大学附属医院眼科,四川成都610072

出　　处：《昆明医科大学学报》2012年第10期19-22,共4页Journal of Kunming Medical University

基　　金：云南省自然科学基金资助项目(2011FZ257)

摘　　要：目的探讨凉血活血药物对视网膜新生血管的干预及其作用机理.方法通过氧诱导C57BL/6幼鼠的方法建立视网膜新生血管的动物模型.随机分为正常对照组、模型对照组、凉血活血药物高、低剂量组.计数视网膜切片中突破血管内界膜的内皮细胞核数;免疫组织化学检测HIF-1和NF-κB在视网膜中的表达情况.结果氧诱导后,幼鼠血管内皮细胞核数目明显增加,HIF-1、NF-κB明显上调,与正常对照组比,差异有统计学意义(P<0.05),提示视网膜新生血管动物模型成功;各治疗组血管内皮细胞核数目减少、HIF-1、NF-κB下调,与模型组比,差异有统计学意义(P<0.05);提示凉血活血药物对视网膜新生血管有抑制作用.结论凉血活血药物可抑制视网膜新生血管的形成.Objective To study the intervention of liangxuehuoxue drugs on retinal neovascularization and the mechanism of action.Methods Retinal neovascularization model was established in young C57BL/6 mice by hyperoxia induction.Mice were divided randomly into 4 groups：normal control group,model group,liangxuehuoxue drugs high dose group and low dose group.The number of nucleus of endothelial cells which broke through the endangium in retinal section,and the expression of HIF-1 and NF-κB in retina was detected by immunohistochemistry.Results Comapred with normal control,after hyperoxia induction,the number of nucleus of endothelial cells significantly increased,and the expression of HIF-1 and NF-κB was upregulated,and the difference was statistically significant（P〈0.05）,suggesting the retinal neovascularization model was established successfully.Compared with model group,in liangxuehuoxue drugs groups,the number of nucleus of endothelial cells significantly decreased,and the expression of HIF-1 and NF-κB was downregulated,and the difference was statistically significant（P〈0.05）.Conclusion Liangxuehuoxue can inhibit the retinal neovascularization,and the mechanism may be related to downregulating the expression of HIF-1 and NF-κB related to the angiogenesis.

关 键 词：视网膜新生血管 凉血活血 HIF-1 NF-ΚB 

分 类 号：R774.1[医药卫生—眼科]