STAT-3与Enolase-1在乳腺癌组织中的表达及意义  

Expression and Clinical Signifiance of STAT-3 and Enolase-1 in Breast Cancer

作　　者：杨洁[1] 叶双梅[1] 蒋学锋 马全富[1] 阚淳一[1] 杨丽兰 卢运萍[3] 王世宣[1] 马丁[1] 吴明富[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430030 [2]深圳市南山医院妇产科 [3]华中科技大学同济医学院附属同济医院肿瘤生物医学中心

出　　处：《肿瘤防治研究》2012年第12期1451-1455,共5页Cancer Research on Prevention and Treatment

基　　金：教育部新教师基金资助项目(200804871056)

摘　　要：目的探讨乳腺癌组织中信号转导及转录活化因子3(STAT-3)和糖酵解酶烯醇化酶(Enolase-1)的表达与雌孕激素受体、淋巴结转移、临床分期、病理分级等的关系。方法免疫组织化学SP法检测126例乳腺癌和26例乳腺良性病变组织中STAT-3与Enolase-1的表达情况,并分析他们与临床病理参数之间的关系。结果乳腺癌组织中STAT-3和Enolase-1的阳性表达率分别为82.5%和77.8%,明显高于乳腺良性病变组织(11.5%和7.7%),差异均有统计学意义(χ2=42.416,P<0.05;χ2=57.211,P<0.05);在有淋巴结转移的乳腺癌组织中阳性表达率分别为85.7%和90.5%,高于无淋巴结转移组,差异均有统计学意义(χ2=9.184,P<0.05;χ2=11.014,P<0.05)。相关分析表明STAT-3和Enolase-1的表达呈正相关。在雌激素受体阳性(ER+)和/或孕激素受体阳性(PR+)或表皮生长因子受体2阳性(HER-2+)乳腺癌组织中,有淋巴结转移组中STAT-3和Enolase-1的表达水平均明显高于无淋巴结转移组,差异均有统计学意义(P<0.05)。结论 STAT-3与Enolase-1的表达与乳腺癌的发生发展及侵袭转移相关,且二者有协同作用(r=0.379,P<0.05)。在ER+和(或)PR+或HER-2+乳腺癌组织中,STAT-3和Enolase-1的高表达与淋巴结转移相关。在高表达STAT-3的乳腺癌组织中Enolase-1表达也较高,其联合检测可作为判断乳腺癌恶性程度、评价预后及指导治疗的一项评估指标。Objective To investigate the expression of STAT-3 and Enolase-1 in breast cancer and its relationship with Estrogen receptor status, Progesterone receptor status, lymph node metastasis, clinical stages and histological grading. Methods The expression of STAT3 and Enolase-I were examined by immunohistochemical streptavid-peroxidase（S-P）assay in 126 cases of breast cancer and 26 cases of benign breast disease. And the significance of its expression was analyzed with clinicopathologic parameters. Results The expression rates of STAT-3（77.8%）and Enolase-1 （82.5%）in breast cancer were significantly higher than that in benign breast disease（11.5 % vs. 7.7 % ）and the expression of two proteins were positively correlated（X2 = 42. 416,P〈0.05 ;）C2 = 57.21 l, P〈0.05）. The expression of STAT 3 and Eno- lase-I in breast cancer has no significant difference in age,tumor size,histologic grading and clinical sta ges. Epression of STAT3 （85.7 % ）and Enolase-1 （90.5% ）in breast cancer with metastasis is significantly higher than that without metastasis （X2 = 9. 184,P〈0.05;）X2 = 11. 014,P〈0.05）. There was a signifi cant correlation between the high expression of STAT-3 and axillary lymphatic metastasis in comparisons between positive estrogen and/or progesterone receptor expression and Her-2 positive expression（P〈0. 05）. And there was a significant correlation between the high expression of Enolase-I and axillary lymphatic metastasis in comparisons with positive estrogen or positive progesterone receptor expression and Her-2 positive expression（P%0.05）. Conclusion Both STAT-3 and Enolase 1 may be involved in the tu- morgenesis development and metastasis of breast cancer. The high expression of STAT-3 and Enolase-1 in estrogen receptor or/and progesterone receptor or Her-2 positive breast cancer was associated with a high possibility of lymph node metastasis. The detection of STAT-3 and Enolase 1 may be helpful in the diagnosis and prognosis of breast cancer.

关 键 词：乳腺肿瘤 淋巴结转移 STAT-3 Enolase-1 

分 类 号：R737.9[医药卫生—肿瘤]