Toll样受体9和低氧诱导因子1α在胰腺癌中的表达及其意义  

Expression and significance of TLR9 and HIF-lα in pancreatic cancer

作　　者：吴汉青[1] 王博[2] 范平[2] 吴河水[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院急诊外科,湖北武汉430022 [2]华中科技大学同济医学院附属协和医院胰腺外科,湖北武汉430022

出　　处：《中华胰腺病杂志》2012年第6期388-391,共4页Chinese Journal of Pancreatology

基　　金：国家自然科学基金（30200272）

摘　　要：目的研究Toll样受体9（TLR9）和低氧诱导因子1仪（HIF-let）在胰腺癌组织中的表达，探讨其临床意义。方法采用实时定量PCR法、蛋白质印迹法及免疫组织化学法检测30例胰腺癌组织及其相邻癌旁组织、10例正常胰腺组织中TLR9和HIF-1et的表达，分析两者表达的相关性、与肿瘤临床病理特征的关系以及对患者生存时间的影响。结果胰腺癌TLR9mRNA及HIF-1etmRNA表达量分别为正常胰腺组织的2．32（1．41～3．22）倍和2．26（1．62～2．89）倍，癌旁组织表达量分别为正常胰腺组织的1．23（1．18～1．28）倍和1．36（1．17～1．55）倍，胰腺癌的表达量显著高于癌旁组织（t=2．642，P=0．023；t=4．076，P=0．001）。胰腺癌组织TLR9和HIF-let蛋白阳性表达率分别为73．3％（22／30）和70．0％（21／30），癌旁组织分别为33．3％（10／30）和36．7％（11／30），正常胰腺组织分别为20％（2／10）和10％（1／10），呈递减趋势（χ2=13．99，P=0．001；0=13．15，P=0．001）。胰腺癌组织TLR9mRNA与HIF．1etmRNA表达呈正相关（r=0．537，P=0．003），TLR9和HIF-1α蛋白阳性表达率亦呈正相关（r=0．511，P=0．001）。胰腺癌组织TLR9和HIF-1d表达与肿瘤分化程度、TNM分期、淋巴结转移呈正相关，与患者的生存时间呈负相关。结论胰腺癌组织TLR9和HIF-let基因均高表达，且与肿瘤细胞的恶性生物学行为及患者预后差有关。Objective To investigate the expression and clinical significance of toll-like receptor 9 （TLR9） and hypoxia inducible factor lα （HIF-lα） in pancreatic cancer. Methods The real-time RT-PCR technique, western blot method and immunohistochemical method were used to examine the expressions of TLR9 and HIF-lα in 30 samples of pancreatic cancer, para-cancerous tissues, and 10 samples of normal pancreatic tissues. The relationship between TLR9 and HIF-1α was determined, and the correlations between their expressions and clinicopathological parameters were measured, and the impact on survival was detected. Results The levels of TLR9 mRNA and HIF-lα mRNA expression in human pancreatic cancer tissues was 2.32（ 1.41 ～ 3.22） and 2.26 （ 1.62 ～ 2.89 ） folds as many as that in normal tissues. The levels of TLR9 mRNA and HIF-lα mRNA expression in para-cancerous tissues were 1.23 （1.18 ～ 1.28 ） and 1.36 （1.17 ～ 1.55） folds as many as that in normal tissues. The expressions in human pancreatic cancer tissues were significantly higher than those in para-cancerous tissues （ t = 2. 642, P = 0. 023 ; t = 4.076, P = 0.001 ）. The positive rates of TLR9 and HIF lα protein were 73.3% （22/30） and 70.0% （21/30）, and the corresponding values were 33.3% （10/30） and 36.7% （11/30） in para-cancerous tissues, while the corresponding values were 20% （2/10） and 10% （1/10） in normal tissues, which showed a decreasing trend （χ2 = 13.99. P =0. 001：χ2 = 13.15, P =0. 001 ）. The expressions of TLR9 mRNA in human pancreatic cancer tissues was positively associated with HIF loL mRNA （ r = 0.537, P = 0. 003 ）. The expressions of TLR9 protein was also positively associated with HIF 1 o~ protein （ r = 0.511, P = 0.001 ）. The expressions of TLR9 and HIF 1 α were positively correlated with the degree of tumor differentiation, TNM staging and lymph node metastasis, but were negatively correlated with the survival. Conclusions TLR9 and HIF-1α are over-expressed in pancreatic ca

关 键 词：胰腺肿瘤 TOLL样受体9 低氧诱导因子1Α 

分 类 号：R735.9[医药卫生—肿瘤]