灵芝孢子粉对戊四氮活化海马神经细胞bax表达的研究  

Study of Ganoderma lucidum spores on pentylenetetrazol activation of hippocampal neurons bax expression

作　　者：张金波[1] 王淑秋[1] 张淑红[1] 金岳雷[1] 朱金玲[1] 刘爽[1] 

机构地区：[1]佳木斯大学基础医学院,黑龙江佳木斯154007

出　　处：《中国优生与遗传杂志》2012年第12期27-28,共2页Chinese Journal of Birth Health & Heredity

基　　金：黑龙江中医药科研项目(编号:ZHY10-W63)

摘　　要：目的本实验研究灵芝孢子粉对戊四氮活化大鼠海马神经细胞bax表达变化的影响,进一步探讨灵芝孢子粉的作用机制和癫痫与海马神经细胞凋亡调控基因之间的关系。方法通过制备癫痫模型和RT-PCR检测正常对照组、癫痫模型组和灵芝孢子粉用药组bax的表达。结果癫痫模型组和灵芝孢子粉用药组bax的表达较正常对照组(0.17±0.04)均升高;其中癫痫模型组(0.66±0.09)bax的表达水平与对照组(0.17±0.04)比较明显升高(P<0.01),灵芝孢子粉用药组(0.47±0.1)bax的表达水平与癫痫模型组比较明显降低(P<0.01),差异有统计学意义。结论本研究结果证实,在PTZ点燃癫痫后,模型组和治疗组促凋亡基因bax表达较正常对照组显著升高,表明bax基因在细胞凋亡的调控过程中起到促进作用,而给予灵芝孢子粉治疗后,bax的表达与模型组比较显著降低,提示灵芝孢子粉有效成份能充分作用于脑组织,可以调控bax的表达,借以发挥抗凋亡的神经保护作用。Objective：This study is Ganoderma lucidum spores on pentylenetetrazol activation of rat hippocampal neurons bax expression changes to further explore the relationship between the action mechanism of the Ganoderma lucidum spores and epilepsy with apoptosis regulatory genes of hippocampal neural.Methods：Through the preparation of the epileptic model and RT-PCR to detect of bax expression in the normal control group,epilepsy model group and Ganoderma spore powder medication group.Results：Bax expression in epilepsy model group and Ganoderma lucidum spores medication group were higher than in the normal control group（0.17 ± 0.04）;including bax expression level in epilepsy model group（0.66 ± 0.09） was significantly higher than in the control group（0.17 ± 0.04）（P0.01）,bax expression level in Ganoderma spore powder medication group（0.47 ± 0.1） were significantly lower than in epilepsy model group（P0.01）,the difference was statistically significant.Conclusion：The results of this study confirmed that model group and the treatment group of bax expression were significantly higher than the normal control group after PTZ kindled,it show that bax genes play a catalytic role in the regulation of apoptosis process.Bax expression were significantly lower than model group after given Ganoderma lucidum spores treatment.It point active ingredients of Ganoderma spore powder can fully act on the brain tissue and regulate the expression of bax in order to play the role of anti-apoptotic neuroprotection.

关 键 词：癫痫 BCL-2基因家族 灵芝孢子粉 

分 类 号：R742.1[医药卫生—神经病学与精神病学]