棉花曲叶病毒外壳蛋白基因启动子的表达方式  

Expressing Pattern of Coat Protein Gene Promoter from Cotton Leaf Curl Virus

作　　者：谢迎秋[1] 朱祯[1] 吴茜[1] 徐鸿林[1] 

机构地区：[1]中国科学院遗传研究所植物遗传操作实验室,北京100101

出　　处：《高技术通讯》2000年第6期1-4,共4页Chinese High Technology Letters

基　　金：863计划!(Z 1 7 0 1 0 1;BH 0 2 0 1 0 1 );国家自然科学基金!(ZJY A 0 7)资助项目

摘　　要：通过农杆菌介导的转基因烟草研究表明 ,棉花曲叶病毒 (CLCuV)外壳蛋白基因 (CP)启动子驱动的GUS基因表达活性不仅存在于韧皮部 ,而且出现在叶肉组织和根尖分生组织中 ;基因枪轰击的瞬时表达研究表明 ,AC2激活后的CP启动子在叶肉及叶脉均有表达活性 ,暗示该启动子为近组成型的启动子。The promoter fragment of coat protein gene(CP) from cotton leaf curl virus w as f used to the GUS reporter gene and transferred to tobacco by Agrobacterium-med iat ed transformation to test promoter expression. Results showed that CP prom oter w as active not only in phloem, but also in mesophyll and root tip meristem. Trans ient expression by particle bombardment demonstrated that activated CP promo ter by AC2 was active in both leaf vein and mesophyll, suggesting that CPpromot er was a near constitutive promoter.

关 键 词：棉花 曲叶病毒 启动子 基因工程 DNA 

分 类 号：S435.62[农业科学—农业昆虫与害虫防治]