粒径对微胶囊成膜及微囊化细胞生长代谢的影响  

Effect of Diameter on Microcapsule Membrane Formation Encapsulated Cells Proliferation and Metabolism

作　　者：马颖[1,2] 张英[1] 赵伟[1,2] 陈立[1,2] 于炜婷[1] 郭昕[1] 马小军[1] 

机构地区：[1]中国科学院大连化学物理研究所生物医学材料工程组,辽宁大连116023 [2]中国科学院研究生院,北京100039

出　　处：《现代生物医学进展》2012年第31期6006-6010,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(21076207;51103157)

摘　　要：目的:考察粒径对微胶囊强度及微囊化细胞生长代谢的影响,为制备性能优良的生物微胶囊提供实验依据。方法:制备不同粒径的凝胶珠,测定其在相同成膜条件下的球磨强度,进而用台盘蓝拒染法测定微囊化细胞的增殖及活率。结果:小粒径的微胶囊具有更厚的微囊膜及更高的球磨强度,另外小粒径微胶囊培养细胞能够获得更多的细胞数(350μm,570μm和900μm微囊内的细胞数量分别为:5.67×107、4.71×107和3.89×107/mL microcapsule,P<0.05)及更高的细胞活率(350μm、570μm和900μm微胶囊的细胞活率分别为:83.70%、67.64%和75.73%,P<0.05)。结论:粒径能影响微胶囊的强度及微囊化细胞的生长、代谢。Objective： To investigate the effect of diameter on microcapsule membrane formation, encapsulated cells proliferation and metabolism for desired microcapsules preparation. Methods： Gel beads with different diameters were coated with membrane under three sets of identical conditions to prepare microcapsules. Bead agitation was used to measure the microcapsule strength and trypan blue staining method was used to measure the encapsulated cells proliferation. Results： Microcapsules with small diameter obtained higher strength and encapsulated cells within small microcapsules obtained higher cell number （Cell number in 350 μm, 570 μm and 900 μm diameter was 5.67× 10^7, 4.71 × 10^7 and 3.89× 10^7/mL respectively, P〈0.05） and higher viability （Cellular viability in diameter of 350 μm, 570 μm and 900 μm was 83.70 %, 67.64 % and 75.73 % respectively, P〈0.05）. Conclusion： The diameter size may be a very significant factor on the microcapsule strength and encapsulated cells proliferation and metabolism.

关 键 词：微胶囊 粒径 强度 细胞生长 

分 类 号：Q78[生物学—分子生物学] Q812