NR2B mRNA在骨癌痛小鼠的前扣带回和脊髓中表达含量变化  被引量：1

THE EXPRESSION OF NR2B MRNA IN THE ANTERIOR CINGULATE CORTEX AND L_(4-6) SPINAL CORD IN THE BONE CANCER PAIN MICE</em> <p class='article-op'> <span id="bdshare" class="bdshare_t bds_tools get-codes-bdshare baidufx"><span><span class="bds_more" style="display: block; width: 62px; height: 29px; background: url(/images/share.gif) no-repeat!important; padding: 0;"></span></span></span> </p> <p class="article-source"> <a href ='/article/read.aspx?id=44256906' class='btnTitle btnIsView' target='_blank'><img alt='在线阅读' src='/images/btn-read.png'/></a><a class='btnTitle btnIsView' href="javascript:showdown('sK3cSlBFj5Lk4wQqkQi19NbmIlREISH8nYFpD5y0FWc=')"><img alt="下载全文" src="/images/btn-down.png" /></a> <input id="hiddowninfo" type="hidden" value="sK3cSlBFj5Lk4wQqkQi19NbmIlREISH8nYFpD5y0FWc="/> </p> <div class="collect" style="display: none"> <h5>我的收藏</h5> <div class="cnt"> <p> 名 称：<input type="text" name="collTitle" id="collSumTitle" /> </p> <p> 注 释：<input type="text" name="collRemark" id="collSumRemark" /> </p> <p style="text-align: center;"> <a href="javascript:void(0)" onclick="g_collectSumOk();"> <img width="65" height="17" src="/images_new/collect_ok.png" alt="完成" /></a> <a href="javascript:void(0)" onclick="g_collectSumCancel();"> <img width="65" height="17" src="/images_new/collect_no.png" alt="取消" /></a> </p> </div> </div> </div> <form name="wxTransfer" method="post"> <input type="hidden" name="lid" value="" /> <input type="hidden" name="sip" value="" /> <input type="hidden" name="date" value="" /> <input type="hidden" name="co" value="" /> <input type="hidden" name="Title_C" value="" /> <input type="hidden" name="Writers" value="" /> <input type="hidden" name="Name_C" value="" /> <input type="hidden" name="Num" value="" /> <input type="hidden" name="Years" value="" /> <input type="hidden" name="Volumn" value="" /> <input type="hidden" name="GCH" value="" /> <input type="hidden" name="Vol" value="" /> <input type="hidden" name="BookID" value="" /> <input type="hidden" name="SubjectNum" value="" /> <input type="hidden" name="SpecialNum" value="" /> <input type="hidden" name="mirrorName" value="" /> <input type="hidden" name="UserID" value="" /> <input type="hidden" name="UserName" value="" /> <input type="hidden" name="sourceID" value="" /> </form> <script type="text/javascript" src="/scripts/transfer.min.js"></script> <div class="article-detail"> <p class='author'><strong>作　　者：</strong><a href='/writer/article.aspx?id=100000010313476' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e6%a2%85%e5%96%9c%5d' title='梅喜[1]'>梅喜[1]</a> <a href='/writer/article.aspx?id=100000010706716' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e9%bb%84%e4%b8%9c%5d' title='黄东[1]'>黄东[1]</a> <em> </em></p><p class='organ'><strong>机构地区：</strong><a href='/organ/article.aspx?id=3109&subid=&showname=&searchtype=' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=S%3d%e4%b8%ad%e5%8d%97%e5%a4%a7%e5%ad%a6%e6%b9%98%e9%9b%85%e4%b8%89%e5%8c%bb%e9%99%a2%e9%ba%bb%e9%86%89%e7%a7%91' title='[1]中南大学湘雅三医院麻醉科,长沙410000'>[1]中南大学湘雅三医院麻醉科,长沙410000</a> <em> </em></p><p class='from'><strong>出　　处：</strong><a href='/journal/summary.aspx?gch=90857X' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=J%3d%5b%e4%b8%ad%e5%9b%bd%e7%96%bc%e7%97%9b%e5%8c%bb%e5%ad%a6%e6%9d%82%e5%bf%97%5d' title='中国疼痛医学杂志'>《中国疼痛医学杂志》</a>2012年第12期739-741,745,共4页<em>Chinese Journal of Pain Medicine</em></p><p class='abstrack'><strong>摘　　要：</strong>目的:观察骨癌痛小鼠前扣带回(anterior cingulate cortex,ACC)和脊髓中的N-甲基-D-天冬氨酸(N-methyl-D-aspartic acid receptor,NMDA)受体2B亚基(NR2B mRNA)表达含量的变化。方法:选用C57BL/6小鼠75只,随机分为癌痛组、假手术组、正常对照组。骨癌痛组接种肺癌细胞约2×106个;假手术组接种等容积的d-hanks液;正常对照组不予任何处理。分别于术后第6 d、10 d、14 d、18 d和22 d测定机械性触诱发痛,取前扣带回和L4~6段脊髓用于做实时定量PCR检测NR2B mRNA的表达。结果:①骨癌痛组于术后第10 d^22 d出现触诱发痛,机械缩足阈值低于对照组和假手术组(p<0.05)。②实时定量PCR显示:术后第14 d、18 d,骨癌痛组前扣带回NR2BmRNA的表达明显高于对照组和假手术组(p<0.05);术后第10 d、14 d、18 d,22 d,骨癌痛组于脊髓NR2B mRNA的表达明显高于对照组和假手术组(p<0.05);而术后第10 d、14 d、18 d,22 d,假手术组与正常组脊髓和前扣带回NR2B的差异无统计学意义(p>0.05)。结论:骨癌痛组脊髓和前扣带回中的NR2B mRNA含量表达上调,提示在骨癌痛的产生和维持中不仅激活脊髓低位中枢,而且有脑部中枢的参与。<em>Objective： To investigate the expression of NR2B mRNA in the anterior cingulate cortex （ACC） and L4-6 spinal cord in the bone cancer pain mice. Methods： 75 adult male C57BL/6 mice were randomly and assigned into three groups （n = 5）： bone cancer pain group （group C）; sham-operated group （group O）; normal control group （group N）. On postoperative 6 d, 10 d, 14 d, 18 d and 22 d after operation, the mechanical allodynia and the expression of NR2B mRNA in the L4-6 spinal cord and ACC were evaluated with the mechanical pain threshold and fluorescence quantitative RT-PCR respectively. Results： Compared with group O and group N, mechanical pain threshold in group C was decreased at 10 d, 14 d, 18 d and 22 d after operation （P 〈 0.05）. Compared with group O and group N, the expression of NR2B mRNA in ACC was increased at 14 d and 18 d after operation （P 〈 0.05）. Compared with group O and group N, the expression of NR2B mRNA in L4-6 spinal cord was increased at 10 d, 14 d, 18 d and 22 d after operation （P 〈 0.05）. Conclusion： The expressions of NR2B mRNA in ACC and L4-6 spinal cord are increased in bone cancer pain mice. These suggest that the generation and maintenance of bone cancer pain is related to both the spinal cord and the ACC.</em></p><p class='subject'><strong>关 键 词：</strong><a href='/subject/article.aspx?id=7563276' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5bNR2B%5d' title='NR2B'>NR2B</a> <a href='/subject/article.aspx?id=6141305' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e9%aa%a8%e7%99%8c%e7%97%9b%5d' title='骨癌痛'>骨癌痛</a> <a href='/subject/article.aspx?id=4250568' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e5%b0%8f%e9%bc%a0%5d' title='小鼠'>小鼠</a> <a href='/subject/article.aspx?id=5732369' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e8%84%8a%e9%ab%93%5d' title='脊髓'>脊髓</a> <a href='/subject/article.aspx?id=3075211' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e5%89%8d%e6%89%a3%e5%b8%a6%e5%9b%9e%5d' title='前扣带回'>前扣带回</a> </p><p class='class'><strong>分 类 号：</strong><a href='/zk/search.aspx?key=C%3dR738' title='R738'>R738</a>[<a href = '/domain/article.aspx?id=503' >医药卫生—肿瘤</a>] </p> </div> <div id="content_wzxg" name="wzxg" class="article-graph"> <object width="695" height="250" classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" id="FlashID"> <param value="../flash/relation.swf" name="movie"> <param value="high" name="quality"> <param value="opaque" name="wmode"> <param value="6.0.65.0" name="swfversion"> <param value="dataUrl=/ajax/articlerelativegraph.ashx?id=44256906" name="flashVars"> <embed width="695" height="250" src="../flash/relation.swf" type="application/x-shockwave-flash" quality="high" wmode="opaque" flashvars="dataUrl=/ajax/articlerelativegraph.ashx?id=44256906"> </embed> </object> </div> <div class="article-relate-list"> <div class="relate-list"> <p id="ckwx_br"> </p> <h2 name="ckwx" id="ckwx">参考文献：</h2> <div id="listckwx"> <p class='submit'> <img src='/images/loading.gif' width='16' height='16' /> 正在载入数据... </p> </div> </div> <div class="relate-list"> <p id="2jckwx_br"> </p> <h2 name="2jckwx" id="2jckwx">二级参考文献：</h2> <div id="list2jckwx"> <p class='submit'> <img src='/images/loading.gif' width='16' height='16' /> 正在载入数据... </p> </div> </div> <div class="relate-list"> <p id="ohwx_br"> </p> <h2 name="ohwx" id="ohwx">耦合文献：</h2> <div id="listohwx"> <p class='submit'> <img src='/images/loading.gif' width='16' height='16' /> 正在载入数据... </p> </div> </div> <div class="relate-list"> <p id="yzwx_br"> </p> <h2 name="yzwx" id="yzwx">引证文献：</h2> <div id="listyzwx"> <p class='submit'> <img src='/images/loading.gif' width='16' height='16' /> 正在载入数据... </p> </div> </div> <div class="relate-list"> <p id="2jyzwx_br"> </p> <h2 name="2jyzwx" id="2jyzwx">二级引证文献：</h2> <div id="list2jyzwx"> <p class='submit'> <img src='/images/loading.gif' width='16' height='16' /> 正在载入数据... </p> </div> </div> <div class="relate-list"> <p id="tbywx_br"> </p> <h2 name="tbywx" id="tbywx">同被引文献：</h2> <div id="listtbywx"> <p class='submit'> <img src='/images/loading.gif' width='16' height='16' /> 正在载入数据... </p> </div> </div> <div class="relate-list"> <p id="xgqklw_br"> </p> <h2 name="xgqklw" id="xgqklw">相关期刊文献：</h2> <div id="listxgqklw"> <p class='submit'> <img src='/images/loading.gif' width='16' height='16' /> 正在载入数据... </p> </div> </div> </div> </div> <div class="article-about"> <div class="about-box"> <h5 class="name">相关的主题<tt><a class="hide" href="#"></a></tt></h5> <div class="about-list"> </div> </div> <div class="about-box"> <h5 class="name">相关的作者对象<tt><a class="" href="#"></a></tt></h5> <div class="about-list" style="display: none;"> </div> </div> <div class="about-box"> <h5 class="name">相关的机构对象<tt><a class="" href="#"></a></tt></h5> <div class="about-list" style="display: none;"> </div> </div> </div> </div> </div> <script type="text/javascript" src="/scripts/articlerelation.min.js"></script> <script type="text/javascript" src="/scripts/articlerightrelation.min.js"></script> <script type="text/javascript" src="/scripts/articledown.min.js"></script> <div class="layer-box download" id="downloadlayer" style="display: none"> <div class="close"> <a onclick="closedown()" href="javascript:void(0)"> <img src="/images/layer-close.png" width="21" height="21" alt="" /> </a> </div> <h5 class="layer-title">下载全文</h5> <div id="downloadinfo"></div> </div> <div class="layer-box download" id="downloadlayer2" style="display:none;min-height:100px;width:500px;"> <p> <img src="/images/loading2.gif" width="64" height="64"> <span style="font-size:20px;vertical-align:middle;padding-bottom:50px;">正在链接到云南高校图书馆文献保障联盟下载...</span> </p> </div> <script id="bdshare_js" data="type=tools&uid=0" type="text/javascript"></script> <script type="text/javascript"> $(function () { var rd = GetQueryString("rd"); 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