Role of TGF-β1/Smads pathway in carotid artery remodeling in renovascular hypertensive rats and prevention by Enalapril and Amlodipine  

作　　者：Jian-Ling Chen Qian-Hui Shang Wei Hu Chan Liu Wan-Heng Mao Hua-Qing Liu 

机构地区：[1]Institute of Clinical Medicine, Zunyi Medical College, Zunyi 563003, Guizhou Province, China [2]Department of Cardiology, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China [3]Department of Pathology, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China

出　　处：《Journal of Geriatric Cardiology》2012年第2期185-191,共7页老年心脏病学杂志（英文版）

摘　　要：Objective To investigate the role of transforming growth factor-β1 （TGF-β1）, Smad2/3 and Smad7 expressions in carotid artery remodeling in renovascular hypertensive rats, and also the therapeutic effect of Enalapril and Amlodipine. Methods The renovascular hypertensive rat （RHR） models with ＂two-kidney and one-clip＂ were established, including model group （n = 6）, sham-operated group （n = 6）, Enalapril group （10 mg/kg per day, n = 6）, Amlodipine group （5 mg/kg per day, n = 6） and combination group （Amlodipine 2.5 mg/kg per day ＋ Enalapril 5mg/kg per day, n = 6）. The medication were continuous administrated for six weeks. Carotid artery morphological and structural changes in the media were observed by HE staining, Masson staining and immuno histochemical staining. Media thickness （MT）, MT and lumen diameter ratio （MT/LD）, and the expression levels of media a-smooth muscle actin （α-actin）, proliferating cell nuclear antigen （PCNA）, TGF-β1, phosphorylated Smad2/3 （p-Smad2/3） and Smad7 in carotid arteries were measured. Results The media of carotid arteries in RHR model group was significantly thickened, the volume of smooth muscle cell was increased, and the array was in disorder; MT, MT/LD, the proliferation index of smooth muscle cell and collagen fiber area percentage of carotid arteries in the model group were significantly higher than those in the sham-operated group （P 〈 0.01）. Compared to sham-operated group, the model group had significantly higher expressions of TGF-β1 and p-Smad2/3 （P 〈 0.05） and lower Smad7 expression. Both Enalapril and Amlodipine improved smooth muscle hypertrophy and collagen deposition, reduced RHR carotid MT, MT/LD, proliferation index of smooth muscle cell, collagen fiber area percentage and the expressions of TGF-β1 and p-Smad2/3 （P 〈 0.05）, increased Smad7 expression （P 〈 0.05）. Moreover, the combination treatment of Enalapril and Amlodipine had significantly better effects than single Amlodipine

关 键 词：HYPERTENSION Vascular remodeling Transforming growth factor-β1 P-SMAD2/3 SMAD7 ENALAPRIL AMLODIPINE 

分 类 号：Q959.837[生物学—动物学] TQ460.6[化学工程—制药化工]