抑癌基因Mxi1在白血病细胞中表达的研究  

Study on the expression of the tumor suppressor gene Mxil in leukemia cells

作　　者：索晓慧[1] 栗金波[1] 李洪军[1] 高建军[1] 崔桂荣[1] 郭玮[1] 张建伟[1] 

机构地区：[1]河北省邯郸市第一医院血液内科,056002

出　　处：《疑难病杂志》2012年第12期933-935,共3页Chinese Journal of Difficult and Complicated Cases

摘　　要：目的分析抑癌基因Mxil在白血病发病中的作用。方法利用逆转录—聚合酶链反应(RT-PCR)检测初治急性白血病(AL)患者26例骨髓单个核细胞,经治疗达完全缓解的AL(AL-CR)患者23例、健康对照者30名外周血单个核细胞和2种髓系白血病细胞株(KG1、K562)Mxil基因表达情况。结果所有标本中均可检测到Mxi1基因的表达,Mxi1 mRNA在初治AL患者的表达水平平均为0.531±0.205,在K562和KG1细胞株中的表达水平平均为0.613±0.223和0.628±0.239,均明显低于健康对照组的0.923±0.187,差异有统计学意义(P<0.05);而AL-CR患者的表达水平为0.812±0.243,明显高于初治AL患者(P<0.05),接近健康对照组(P>0.05)。结论白血病患者细胞中Mxi1基因表达水平下降,提示其可能与白血病的发病有关。Objective To analyze Mxil gene＇s role in leukemic cells.Methods Expression and mutation of Mxil gene in bone marrow mononuclear cells（BMMNC） from 26 patients with de novo acute leukemia（AL）,23 AL patients achieved complete responses（AL-CR） after treatment,and in peripheral blood mononuclear cells（PBMNC） from 30 healthy volunteers as normal control,as well as 2 human leukemic cell lines（KG1,K562） were detected by reverse transcription-polymerase chain reaction（RT-PCR）.Results Among all cases,Mxil gene could be detected.The average expression level of the Mxil gene was 0.531±0.205 in patients with de novo AL,0.613±0.223 and 0.628±0.239 on average in the human myeloid cell line KG1 and K562,respectively.The levels of Mxil gene in the experimental group were significantly lower than that in the control group（0.923±0.187）.There was significant statistical differences between experimental group and control group（P 0.05）;Meanwhile,levels of Mxil gene in AL-CR patients（0.812±0.243） were significantly higher than de novo AL（P 0.05）,and closer to its level in health group（P 0.05）.Conclusion Mxil gene expression declined in acute myeloid leukemia,it suggests that this gene might be associated with leukemia.

关 键 词：抑癌基因 Mxi1 白血病 

分 类 号：R733.7[医药卫生—肿瘤]